接触性超敏反应小鼠不同部位T细胞亚群的变化  

Experimental Research of Changes of T Cell Subsets in Different Tissues of Contact Hypersensitivity Mice

作　　者：张立[1] 江益平[1] 周联[1] 王丽虹[1] 梁明 

机构地区：[1]广州中医药大学免疫与分子生物学实验室,广东广州510405 [2]无限极(中国)有限公司研发中心,广东广州510665

出　　处：《广州中医药大学学报》2013年第2期191-195,共5页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(编号:30873415);广东省教育部产学研结合项目(编号:2010A090200041)

摘　　要：【目的】比较接触性超敏反应(CHS)小鼠不同部位CD4+/CD8+T细胞以及辅助性T细胞(Th1/Th2)、细胞毒性T细胞(Tc1/Tc2)的平衡状态。【方法】以二硝基氟苯(DNFB)致敏和攻击BALB/c小鼠,建立CHS模型。采用三色流式细胞术分析CHS小鼠脾脏(SP)、耳后淋巴结(RLN)、小肠派氏结(PPs)和肠系膜淋巴结(MLN)中CD4+T细胞和CD8+T细胞的变化及其胞内细胞因子干扰素-γ(IFN-γ)与白细胞介素-4(IL-4)的表达水平,观察CHS小鼠不同部位Th1/Th2、Tc1/Tc2比例的变化。【结果】与正常组比较,CHS组RLN与SP中CD4/CD8比值显著下降(均P<0.01),RLN中以Tc1与Th1占优势,SP中以Tc1与Th2占优势,PPs中Tc1细胞比例、CD4/CD8比值显著上升(均P<0.05),MLN中各指标无显著性差异。【结论】DNFB诱发的CHS小鼠机体总体免疫平衡向Th1类偏移,Tc1(CD8+IFN-γ+)细胞是主要的效应细胞。Objective To compare the changes of CD4 ＋/CD8 ＋ T cell subsets and intracellular cytokines of Thl/ Th2, Tcl/Tc2 cells in different tissues of contact hypersensitivity （CHS） mice. Methods BALB/c mice were sensitized and challenged with dinitrofluorobenzene （DNFB） to induce CHS model. Trichromatism flow cytometry was adopted to detect the changes of CD4＋ and CD8 ＋ T cell subsets in the spleen （ SP）, retroauricular lymph nodes （ RLN）, intestinal Peyer＇ s patches （PPs） and mesenteric lymph nodes （MLN） as well as the expression of intracellular cytokines of interferon gamma （IFN--γ） and interleukin 4 （IL-4） cells. The changes of Thl/Th2 and Tcl/Tc2 ratio in different tissues of CHS mice were also observed. Results Compared to the normal group, CD4/ CD8 ratio decreased significantly in RLN and SP of CHS mice （P 〈0. 01 ）. Tcl and Thl cells were dominated in RLN of CHS mice, Tcl and Th2 cells dominated in SP, Tcl cells percentage and CD4/CD8 ratio significantly increased in PPs （ P 〈 0. 05 ）. There was no significant difference in the indexes of MLN. Conclusion The murine contact hypersensitivity is mainly mediated by Tcl （ CD8 ＋ IFN-γ＋ ） effector cells, and the intestinal mucosal immunity and system immunity in DNFB-induced CHS mice are dominated by Thl cells.

关 键 词：接触性超敏反应 T细胞亚群 细胞因子 流式细胞术 肠黏膜 免疫学 

分 类 号：R392.8[医药卫生—免疫学]