精浆天门冬氨酸氨基转移酶及其线粒体同工酶检测与精子膜功能完整性的关系  

Determination of seminal AST and m-AST activities and their relationship with functional integrity of human sperm membrane

作　　者：彭明[1] 顾向明[1] 梁有娣[1] 

机构地区：[1]广州中医药大学附属中山市中医院检验科,广东中山528401

出　　处：《检验医学与临床》2013年第5期515-517,共3页Laboratory Medicine and Clinic

基　　金：广东省中山市科技计划项目资助课题(NO.20102A061)

摘　　要：目的对精浆天门冬氨酸氨基转移酶(AST)及其线粒体同工酶(m-AST)的活性进行检测,并分析AST和m-AST活性与精子膜功能完整性的相关性。方法 122例精液标本,分别检测精浆AST和m-AST活性,用低渗肿胀-伊红Y结合试验(HOS-EY)检测精子头-尾膜完整性,用琥珀酸脱氢酶(SDH)染色法检测精子线粒体SDH,同时分析AST和m-AST活性与精子膜功能完整性的相关性。结果精浆AST与m-AST的活性分别为(238±125)U/L和(147±79)U/L,两者呈明显正相关性(r=0.740,P=0.000)。AST和m-AST活性均与(Ⅰ+Ⅱ+Ⅲ)型精子呈明显正相关性(r=0.443,P=0.013;r=0.691,P=0.000)。AST和m-AST活性均与Ⅳ型精子呈明显负相关性(r=-0.439,P=0.013;r=-0.689,P=0.000)。AST活性与精子SDH阳性率无明显相关性(r=-0.187,P=0.313),而m-AST活性与精子SDH阳性率呈明显负相关性(r=-0.471,P=0.007)。结论精浆AST和m-AST活性与精子膜功能完整性具有相关性,而m-AST活性检测更适合评价精子膜受损程度以及精子线粒体受损状况。Objective To determine the seminal aspartate aminotransferase（AST） and mitochondrial AST（m- AST） activity,and to analyze their relationship with the functional integrity of human sperm membrane. Methods AST and m-AST activities in 122 samples of seminal plasma were measured. The sperm head membrane and tail membrane integrity were detected by the combined hypoosmotic swelling and eosin Y exclusion test（HOS-EY）. The mitochondrial succinate dehydrogenase（SDH） of sperm was detected by the SDH dyeing method and the correlation between seminal AST or m-AST activity with the functional integrity of human sperm membrane. Results The AST and m- AST activities were （ 238±125 ） U/L and （ 147±79 ） U/L respectively, there was significant positive correlation between them（r= 0. 740,P=0. 000）. Both seminal AST and m-AST activities had obvious positive correlation with type Ⅰ and Ⅱ and Ⅲ sperms（r=0. 443,P=0. 013;r=0. 691,P=0. 000）. Both seminal AST and m-AST activities had obvious negative correlation with type IV sperm（r=- 0. 439, P= 0. 013 ; r= - 0. 689, P= 0. 000）. There was no obvious correlation between AST activity and sperm SDH positive rate（r=-0. 187 ,P=0. 313） ,but seminal m-AST activities had significant negative correlation with sperm SDH positive rate（r =- 0. 471, P = 0. 007）. Conclusion The seminal AST and m-AST activities have significant correlation with the functional integrity of human sperm membrane. The determination of m-AST activity is more suitable for evaluating the damage degree of sperm mem- brane and sperm mitochondria.

关 键 词：天门冬氨酸氨基转移酶 同工酶 精子膜功能 完整性 精浆 

分 类 号：R321.1[医药卫生—人体解剖和组织胚胎学]