巴豆生物碱的薄层色谱鉴别研究  

Thin—layer chromatography of total alkaloids in Croton tiglium

作　　者：李晓伟[1] 易智彪[1,2] 曾宝[1,2] 李生梅[1] 唐君苹[1] 袁捷[1,2] 

机构地区：[1]广东省广州中医药大学,广州510000 [2]广东省东莞广州中医药大学中医药数理工程研究院,东莞523808

出　　处：《北方药学》2013年第2期3-4,共2页Journal of North Pharmacy

摘　　要：目的:建立巴豆生物碱的薄层鉴别方法,为巴豆药材的质量评价提供依据。方法:采用TLC法,以巴豆苷和木兰花碱为对照品,以氯仿-甲醇-水(6:4:1,加5滴氨水)为展开系统,碘熏显色。结果:在日光下检视,在两组对照品相应位置上,显出相同颜色的斑点。结论:该方法简便、准确、重现性好,为评价巴豆药材定性鉴别及内在质量提供了科学依据。Objective：To establish a thin layer chromatographic（TLC） method for the Croton alkaloids, and to offer evidence for quality evaluation of Fructus Crotonis. Methods： The determination was done by using TLC. With isoguanosine and magnoflorine as reference, water （6：4：1,5 drops of ammonia） as the develop system was established. After launching and took out air driesand developed with iodine vapor. Results： A same eolor spots in the TLC graph of sample existed at the corresponding positions compared with the controls. Conelusions： The established method is simple and accurate,and have a good repeatability,which will supply evidence for the quality evaluation of medicinal material of Fructus Crotonis.

关 键 词：巴豆 TLC 巴豆苷 木兰花碱 

分 类 号：R284[医药卫生—中药学]