北五味子提取物碱性水解主要木脂素增量及其优化  

Optimizing on Alkaline Hydrolysis and Organic Solvent Extract of Lignans from Schisandra Chinensis

作　　者：马宇亮[1] 李丽丽[1] 周启超[1] 孙曦晓[1] 孙震[1] 张晓楠[1] 马春慧[1] 杨磊[1] 祖元刚[1] 

机构地区：[1]东北林业大学生物资源生态利用国家地方联合工程实验室,黑龙江哈尔滨150040

出　　处：《黑龙江医药》2013年第1期34-36,共3页Heilongjiang Medicine journal

基　　金：公益性行业科研专项经费项目(201304601)资助

摘　　要：采用80%体积分数的乙醇回流提取方法对北五味子活性成分五味子醇甲、五味子酯甲、五味子甲素和五味子乙素进行提取,采用单因素实验考察水解催化剂的影响,确定以氢氧化钠作为催化剂;应用响应面分析对北五味子乙醇提取物碱水解工艺进行了优化,确定用1mol/L的氢氧化钠15mL回流水解2h。然后考察了三氯甲烷、1,2-二氯乙烷、乙酸乙酯和石油醚几种溶剂的萃取效果,确定以1,2-二氯乙烷作为70%浸膏溶液的萃取溶剂。北五味子木脂素的有效成分五味子醇甲、五味子酯甲、五味子甲素和五味子乙素含量分别由原来的150.42、44.02、248.30和514.29mg/kg增加到455.96、58.21、397.69和772.09mg/kg;经验证后总木脂素的回收率可达到165.91%。Extracting the lignans from Schisandra chinensis by 80% Ethanol The effect of hydrolysis catalyzer was investi- gated and sodium hydroxide was the optimum catalyzer. The optimal operating conditions are obtained ： 1 mol/L sodium hy- droxide 15mL, reflexing extraction 2 h with 100℃. Then compared the extractive effect of chloroform, 1,2 -chloroethene, eth- yl acetate and petroleum ether and determined that 1,2 - chloroethene was the solvent of extraction in 70% ethanol The total lignans content of schizandrin, schisantherin A, deoxyschizandrin and γ -schizandrin from the fruit of S. chinensis increased from original 150. 42,44. 02,248. 30 and 514. 29 mg/kg to 455.96,58.21,397.69 and 772.09 mg/kg respectively. The recy- cle of the total lignans gets to 165.91%.

关 键 词：北五味子 木脂素 碱水解 

分 类 号：R284.2[医药卫生—中药学]