贵州矮马与伊犁马基因组的RAPD分析  

Genetic variation of Guizhou pony and Ili horse by RAPD analysis

作　　者：雷红梅[1] 王嘉福[1,2] 田松军 王荣明 黄勇 冉雪琴[2] 

机构地区：[1]贵州大学生命科学学院,贵州贵阳550025 [2]贵州大学动物科学学院,贵州贵阳550025 [3]贵州省紫云县农业局,贵州紫云561600

出　　处：《广东农业科学》2013年第2期139-142,共4页Guangdong Agricultural Sciences

基　　金：贵州省农业攻关项目(黔科合NY字2009-3068;黔科合NY字[2012]3009号);贵州省农业动植物育种专项(黔农育专字2009-021号);贵州省科技创新人才团队建设专项(黔科合人才团队2009-4006);贵州大学研究生创新基金(校农研2012010)

摘　　要：应用RAPD技术对贵州矮马的遗传多样性进行研究,并与伊犁马进行比较。以120条RAPD引物对两个马品种的混合基因组DNA进行筛选,发现6条引物可产生多态性,并用其对84份样品进行扩增,共获得123条带,其中101条为多态性条带。研究结果表明,贵州矮马与伊犁马的Nei指数分别为0.3136和0.3098,两个品种间遗传分化系数为4.74%,说明贵州矮马的遗传变异较伊犁马小,贵州矮马与伊犁马品种间的基因交流很少;计算个体的遗传相似系数并建立了系统发育树,获得9个分支,其中3个分支中两种马的分布比例相近,其他分支由1个马品种独享占主导,第4、5、6支中两种马所占的比例相近,说明两个马品种拥有共同的祖先,在较早的时间分离后单独进化。The genetic variations of Guizhou pony and Ili horse were analyzed by random amplification of polymorphic DNA （RAPD） marker. Six single polymorphic primers were selected from 120 primers. A total of 123 bands were amplified and 101 bands showed plenty of polymorphisms. As analyzed by POPGENE software, Nei＇s genetic diversity of Guizhou pony and Ill horse were calculated to be 0.3136 and 0.3098, respectively. It indicated that the genetic diversity in Guizhou pony was less than that in Ili horse. The proportion of the genetic differentiation among Guizhou pony and Ili horse （GST） was 4.74%, which illustrated that gene exchange between Guizhou pony and Ili horse was very low. The individual genetic similarity coefficient was calculated and the dendrogram was established with NTsys 2.10e software. Eighty-four samples was divided into nine branches in the dendrogram. Only Guizhou pony or Ili horse distributed in six branches while both of them shared the other three branches. It suggested that Guizhou pony and Ill horse might share a common ancestor but separated from each other in the early dates and continued evolution process independently.

关 键 词：RAPD 遗传变异 贵州矮马 伊犁马 

分 类 号：Q78[生物学—分子生物学]