高效液相色谱法测定α-乳白蛋白和β-乳球蛋白  

Determination of α-lactalbumin and β-lactoglobulin by High Performance Liquid Chromatography

作　　者：王莹[1] 屈玉霄[2] 刘春红[2] 冯志彪[2] 

机构地区：[1]东北农业大学食品学院,黑龙江哈尔滨150030 [2]东北农业大学理学院应用化学系,黑龙江哈尔滨150030

出　　处：《食品研究与开发》2013年第2期57-60,共4页Food Research and Development

基　　金：东北农业大学博士基金;黑龙江省青年基金(QC2010029)

摘　　要：采用HPLC法测定牛乳中主要过敏蛋白α-乳白蛋白及β-乳球蛋白的含量,其测定条件为:Agilent-C8(4.6 mm×150 mm,5μm)色谱柱,流动相A为含0.5%三氟乙酸的乙腈,流动相B为含0.1%三氟乙酸的超纯水,采用梯度洗脱的方法,流速1 mL/min,柱温25℃,检测波长280 nm。结果表明,α-乳白蛋白和β-乳球蛋白的回收率均>92%,RSD<5%。该方法适合测定样品中的α-乳白蛋白和β-乳球蛋白。Abstract： A method for the determination of αlactalbumin and β-lactoglobulin in milk products by high performance liquid chromatography has been established. The chromatography conditions as follow： Agilent-Cs column （4.6 mm×150 ram, 5μm）. This method consisted of a linear gradient of the two mobile phases of 0.5 % trifluoroacetic acid in aeetonitrile and 0.1% trifluoroacetic acid in water at a flow rate of 1 mL/min. The column temperature was 25 ℃ and UV detection wavelength was 280 nm. The result showed that recovery percent more than 92 %, the RSD less than 5 %. The method is suitable for contents analysis of α-lactalbumin and β- lactoglobulin in the samples.

关 键 词：高效液相色谱(HPLC) α-乳白蛋白(α-lactalbumin) β-乳球蛋白(β-lactoglobulin) 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程] O657.72[轻工技术与工程—食品科学与工程]