赶黄草对体外诱导的酒精性脂肪肝细胞的影响  

Effect of Chinense Pursh Penthorum on cells of alcoholic fatty liver induced by ethanol in vitro

作　　者：袁叶飞[1] 胡祥宇[1] 欧贤红[2] 

机构地区：[1]泸州医学院药物化学教研室,四川泸州646000 [2]泸州医学院心肌电生理学研究室,四川泸州646000

出　　处：《泸州医学院学报》2013年第1期35-38,共4页Journal of Luzhou Medical College

摘　　要：目的:建立酒精性脂肪肝细胞模型,探讨赶黄草体外对酒精性脂肪肝细胞的影响。方法:采用0.5%乙醇和0.01%的硫酸亚铁(FeSO4)加入L-02正常人肝细胞培养液,建立酒精性脂肪肝细胞模型。采用CCK-8方法筛选赶黄草的最佳作用浓度,利用酒精性脂肪肝细胞模型评价赶黄草体外对酒精性脂肪肝细胞的作用。结果:用含0.5%的乙醇和0.01%的FeSO4的RPMI-1640培养液诱导L-02正常人肝细胞,诱导至第3代,即可使肝细胞产生明显甘油三酯堆积。CCK-8方法筛选赶黄草的最佳作用浓度为40μg.ml-1,40μg.ml-1的赶黄草可显著降低酒精性脂肪肝细胞的甘油三酯。结论:采用0.5%乙醇和0.01%硫酸亚铁混合物体外诱导L-02正常人肝细胞,可短时间内建立酒精性脂肪肝细胞模型,赶黄草有减轻体外诱导的酒精性脂肪肝细胞脂肪变的作用。Objective：To establish alcoholic fatty liver cell model,and to explore the therapeutic effect of Chinese Pursh Penthorum on alcoholic fatty liver in vitro. Methods：The alcoholic fatty liver cell model was established by treating human liver cell L-02 with 0.5% ethanol and 0.01% FeSO4. CCK-8 assay was used to screen the optimal concentration of Chinese Pursh Penthorum. The therapeutic effect of Chinese Pursh Penthorum was e- valuated on alcoholic fatty liver cell model. Results：When L-02 cells were induced to the third generation with 0.5% ethanol and 0.01% FeSO4 in RPMI-1640 medium, triglyceride obviously accumulated in cells. The optimal concentration of Chinese Pursh Penthorum screened by CCK-8 assay was 40μg.ml^-l,under this coneentrarion triglyceride in alcoholic fatty liver cells was significantly reduced. Conclusion：The alcoholic fatty liver cell model can be established by 0.5% ethanol and 0.01% FeSO4 in vitro in a short period of time.Chinese Pursh Penthorum. is helpful to therelief of steatosis of alcoholic fatty liver induced in vitro.

关 键 词：酒精性脂肪肝 细胞模型 赶黄草 

分 类 号：R931[医药卫生—生药学] R575[医药卫生—药学]