经颅注射日本血吸虫卵建立脑型血吸虫病模型  

Preliminary study on establishing an animal model of neuroschistosomiasis by direct injection of Schistosoma japonicum eggs through skull

作　　者：徐嘉[1] 鲁晓杰[1] 王丹[2] 吴明灿[3] 陈世洁[3] 成祥林[4] 李军川[5] 王鹏[1] 

机构地区：[1]江苏省无锡市第二人民医院神经外科,无锡214000 [2]长江大学附属第一医院中心实验室 [3]长江大学附属第一医院神经外科 [4]长江大学附属第一医院科教科 [5]长江大学附属第一医院病理科

出　　处：《中国血吸虫病防治杂志》2013年第1期28-30,共3页Chinese Journal of Schistosomiasis Control

基　　金：江苏省卫生厅血地寄防应用性科研项目(X201117);湖北省卫生厅血吸虫病防治科研项目(XF2008 23;XF06D43)

摘　　要：目的建立一种新的家兔脑型血吸虫病模型以探讨脑型血吸虫病的形成机理及建模的影响因素。方法以家兔为实验对象,分别通过钻孔法和锥颅法经颅直接注射日本血吸虫卵悬液1 m(l1 mg/ml)。观察临床症状,注射后第1周的第3、5天,第2周的第1、3、5天及2周后,取兔脑制作病理切片,观察其病理变化。结果家兔在建模第1～2周即出现厌食、偏瘫、癫痫等多种神经系统症状。脑组织病理切片显示虫卵肉芽肿改变。建模成功率达90%。结论采用经颅直接注射虫卵法成功建立了脑型血吸虫病动物模型,为开展实验研究提供了基础。Objective To establish an experimental model of neuroschistosomiasis and investigate the model establishment factors. Methods Rabbits were used for the animal model and Schistosomajaponicum eggs （ 1 mg/ml） were directly injected into the brain by two ways of a bone drill or needle. The symptoms were observed and in the first and second week and later, the rab- bits＇ brains were removed for pathological examinations. Results One to two weeks after the injection of schistosome eggs, the rabbits had various neurological symptoms such as loss of appetite, hemiparesis, seizure, etc. The pathological analysis showed the schistosome egg granuloma inflammatory reaction among 90% rabbits. Conclusion This new method of direct injection of S. japonicum eggs through skull into the brain provides a good and easy animal model of neuroschistosomiasis.

关 键 词：日本血吸虫病 脑型血吸虫病 动物模型 虫卵肉芽肿 

分 类 号：R532.21[医药卫生—内科学]