胎鼠神经干细胞移植对大鼠脊髓损伤后神经元特异性烯醇化酶和皮质体感诱发电位的影响研究  

Effect of Neural Stem Cells Transplantation on Neuron Specific Enolase and Cortical Somatosensory Evoked Potentials of Rat after Spinal Cord Injury

作　　者：孔令胜[1,2] 靳峰[2] 郭强[2] 张浩[2] 胡亚伟[2] 

机构地区：[1]青岛大学,山东省青岛市266071 [2]济宁医学院附属医院神经外科

出　　处：《中国全科医学》2013年第6期639-643,共5页Chinese General Practice

基　　金：山东省自然科学基金资助项目(ZR2010HM040)

摘　　要：目的研究神经干细胞(NSCs)移植对大鼠脊髓损伤(SCI)后神经元特异性烯醇化酶(NSE)和皮质体感诱发电位(CSEP)的影响。方法将40只SD大鼠随机分为5-溴脱氧尿嘧啶核苷(Brdu)+NSCs组、NSCs组、SCI组和假手术组(Sham组),采用电控SCI打击装置制作模型,Brdu法标记处于对数生长期的NSCs,SCI后即刻进行NSCs移植。CSEP监测神经电生理的恢复,苏木素-伊红(HE)染色观察大鼠脊髓细胞形态的变化,免疫组织化学法观察Brdu标记NSCs的存活、迁移以及NSE的表达。结果 4组间各时间点皮质体感诱发电位-P波(CSEP-P)潜伏期比较,差异均有统计学意义(P<0.01)。Sham组和SCI组各时间点间比较,差异均无统计学意义(P>0.05);而Brdu+NSCs组和NSCs组各时间点间比较,差异均有统计学意义(P<0.01)。其中SCI组、NSCs组和Brdu+NSCs组各时间点CSEP-P潜伏期与Sham组比较,差异均有统计学意义(P<0.01);NSCs组和Brdu+NSCs组除第1天外余各时间点与SCI组比较,差异均有统计学意义(P<0.01);Brdu+NSCs组与NSCs组比较,差异无统计学意义(P>0.05)。4组间各时间点NSE免疫阳性细胞灰度值及阳性细胞数比较,差异均有统计学意义(P<0.01)。Sham组各时间点间比较,差异无统计学意义(P>0.05);而SCI组、Brdu+NSCs组和NSCs组各时间点间比较,差异均有统计学意义(P<0.01);其中SCI组、NSCs组和Brdu+NSCs组各时间点NSE免疫阳性细胞灰度值及阳性细胞数与Sham组比较,差异均有统计学意义(P<0.01);NSCs组和Brdu+NSCs组除第1天外余各时间点与SCI组比较,差异均有统计学意义(P<0.01);Brdu+NSCs组与NSCs组比较,差异无统计学意义(P>0.05)。NSE免疫阳性细胞灰度值及阳性细胞数与CSEP-P潜伏期均呈负相关(r=-0.937,P<0.001;r=-0.956,P<0.001)。结论体外培养的NSCs移植到SCI区域后可存活、迁移,且能明显上调SCI后NSE的表达,并能通过促进NSE的表达而缩短CSEP-P潜伏期,从而促进大鼠后肢功能障碍的恢复。Objective To study the effect of neural stem cells （NSCs） transplantation on neuron specific enolase （NSE） and cortical somatosensory evoked potentials （CSEP） of rat after spinal cord injury （SCI） . Methods SD rats were randomly divided into Brdu ＋ NSCs group, NSCs group, SCI group and sham group. SCI models were induced with electrocircuit controlled spinal cord injuring device. NSCs in logarithmic phase were labeled with Brdu. The NSCs were transplanted into the in- jured site after SCI. HE staining was used to observe the pathological changes of spinal cord tissues, CSEP was used to monitor the recovery of spinal cord and immunohistochemisty method was used to observe NSE and the survival and migration of NSCs labeled by Brdu. Results There were significant differences on the latency of CSEP - P wave at each time point in four groups （ P 〈 0. 01 ）, and there was no significant difference between Sham group and SCI group （ P 〉 0. 05 ）, but there were significant differences between Brdu ＋ NSCs and NSCs group （P 〈 0. 01 ） . Compared with Sham group, there were significant differences on the latency of CSEP - P wave at each time point in SCI, NSCs and Brdu ＋ NSCs groups （P 〈 0. 01 ） ; Except the first day, there were significant differences in NSCs and Brdu ＋ NSCs groups compared with SCI group （P 〈0.01 ） ; there was no signifi- cant difference between NSCs and Brdu ＋ NSCs group （P 〉 0.05） . There were significant differences on the grey level and im- munostaining positive cells of NSE at each time point in four groups （P 〈 0. 01 ） and there were no significant differences at each time point in Sham group （P 〉 0. 05）, but there were significant differences among SCI, NSCs and Brdu ＋ NSCs groups （P 〈 0. 01） , then there were significant differences in SCI, NSCs and Brdu ＋NSCs groups compared with Sham group （P 〈0. 01 ） ;Except the first day, there were significant differences in NSCs and Brdu ＋ NSCs groups compared with SCI

关 键 词：脊髓损伤 干细胞移植 神经元特异性烯醇化酶 皮质体感诱发电位 

分 类 号：R651.21[医药卫生—外科学]