棉花竹试管快繁技术研究  

Rapid Propagation of Fargesia fungosa by Tissue Culture

作　　者：宋李玲[1] 李瑞珺 刘倩倩[1] 林新春[1] 

机构地区：[1]浙江农林大学亚热带森林培育国家重点实验室培育基地,浙江临安311300 [2]浙江省临安市林业局,浙江临安311300

出　　处：《江西农业大学学报》2013年第1期59-62,共4页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金项目(31270677);浙江省自然科学基金重点项目(Z3100366)

摘　　要：以棉花竹(Fargesia fungosa)成熟种胚诱导出的芽作为材料,研究其快速繁殖技术。结果表明:棉花竹的最佳增殖培养基为MS(Murashige and Skoog)+3 mg/L BA(6-苄基腺嘌呤),增殖系数为3.77,芽丛生长旺盛;最佳生根培养基为1/2MS+3 mg/L IBA(吲哚丁酸),生根率达100%,根系较长且粗壮;试管苗移栽至泥炭、蛭石、珍珠岩配制比例为1∶1∶1的混合基质中,成活率高达98%。本研究结果为棉花竹的扩繁和产业化生产提供了技术支撑,对箭竹属其他竹种的组织培养具有重要的参考价值。The buds induced from mature embryos were used as explants to study the rapid propagation technique for Fargesia fungosa. Murashige and Skoog＇ s （MS） medium supplemented with 3 mg/L BA was the optimal medium for bud multiplication. The highest proliferation coefficient was 3.77, and the cluster buds grown well. The suitable rooting medium was 1/2 MS supplemented with 3 mg/L IBA. The rooting rate was up to 100% , and the roots were long and thick. The survival rate of hardening plantlets was 98% after being transferred to a mixture of peat, vermiculite and perlite with a ratio at 1 ： 1 ： 1. The study provides technical support for the propagation and industrialization production of Fargesia fungosa, and also is of important refer- ence value to the tissue culture of other species in the genus.

关 键 词：棉花竹 组织培养 丛生芽 快繁 

分 类 号：S722.37[农业科学—林木遗传育种]