1128例HBV-DNA定量和乙肝血清学标志物定量结果之间的相关性研究  

Study on relationship between the quantitive detection of HBV DNA and serological markers in 1128 hepatitis B patients

作　　者：杨国绘 许文龙[1] 窦琳琳[1] 杨爱平[1] 

机构地区：[1]浙江省浙江萧山医院检验科,浙江萧山311202

出　　处：《中国卫生检验杂志》2013年第2期351-354,共4页Chinese Journal of Health Laboratory Technology

摘　　要：目的:研究HBV-DNA定量和乙肝血清学标志物定量之间的相关性,为确定乙肝检测项目及临床治疗提供科学依据。方法:采用实时荧光定量扩增法(FQ-PCR)检测HBV-DNA,化学发光微粒子免疫分析法(CMIA)检测乙肝血清学标志物。结果:(1)HBV-DNA低载量组(5.0×102copies/ml～9.9×104copies/ml)乙肝大三阳检出率28.5%,乙肝小三阳检出率66.4%;HBV-DNA高载量组(1.0×105copies/ml～9.9×108copies/ml)乙肝大三阳检出率80.3%,乙肝小三阳检出率14.5%,差异均有统计学意义(P<0.01)。(2)142例HBsAg浓度处于线性范围(0.05 IU/ml～250 IU/ml),其中HBV-DNA低载量检出率90.8%;HBV-DNA高载量检出率9.2%,差异有统计学意义(P<0.01)。结论:HBV-DNA与乙肝血清学标志物两者之间互有关联又有区别,两者联合且定量检测能更加全面地反应乙肝病毒感染者的状况。Objective：To study the relationship between the quantitive detection of HBV - DNA and serological markers of hepatitis B, so as to provide scientific basis for making hepatitis B testing projects and clinical treatment. Methods： HBV - DNA was detected by FQ - PCR, the quantities of serological markers of hepatitis B were tested by CMIA. Results ： 1. In lower copies group （5.0 × 102 copies/m[ - 9.9 × 104 eopies/ml） of HBV - DNA, the HB- sAg - positive, HBeAg - positive and HBcAb - positive accounted for 28.5% , the HBsAg - positive, HBeAb - pos- itive and HBcAb - positive accounted for 66. 4% ; in higher copies group （ 1. 0 × 105 copies/ml - 9. 9 × 108 copies/ml）of HBV - DNA, the HBsAg - positive, HBeAg - positive and HBcAb - positive accounted for 80.3% , the HBsAg- positive, HBeAb- positive and HBcAb -positive accounted for 14.5% , the differences all had statistical significance （ P 〈 0.01 ）. 2. HBsAg was linear in the concentration range of 0.05 IU/ml - 250 IU/ml in 142 samples, and the detection rate of HBV- DNA with low copies was 90.8%, while that of HBV -DNA with high copies was 9.2% , the difference had statistical significance （P 〈 0.01 ）. Conclusion： There was statistical significance between the copies of HBV - DNA and the quantities of serological markers of hepatitis B, but they were also different with each other, combination of the two quantitive testing methods could more perfectly indicate the state of patients with semi -hepatitis B infection.

关 键 词：HBV—DNA 乙肝血清学标志物 载量 相关性 

分 类 号：R512.62[医药卫生—内科学]