Diversilab系统用于金黄色葡萄球菌同源性分析  

Homologous analysis of Staphyloccocus aureus by Diversilab System

作　　者：于纪棉[1] 劳华均[2] 李如松[2] 孙萍[2] 张玉琳[1] 

机构地区：[1]宁波卫生职业技术学院,浙江宁波315100 [2]宁波出入境检验检疫局,浙江宁波315012

出　　处：《中国卫生检验杂志》2013年第2期370-371,374,共3页Chinese Journal of Health Laboratory Technology

基　　金：国家质检总局科研项目(2010IK169)

摘　　要：目的:用Diversilab System DNA指纹分型系统(Diversilab系统)绘制金黄色葡萄球菌DNA指纹图谱,分析各菌株间的同源性。方法:对食品中分离到的42株金黄色葡萄球菌提取DNA,进行rep-PCR,用Diversilab系统中的软件对DNA指纹图谱进行同源性分析。结果:实验得到了42株的金黄色葡萄球菌的DNA指纹图谱,可以分成14群,菌株之间的相似性68.9%～99.7%,未表现地区特异性。结论:Diversilab系统具有操作简便、快速、重复性好、分辨率高的特点,可用于黄色葡萄球菌快速分型和溯源。Objective：To draw a DNA fingerprinting of Staphyloccocus aureus strains by Diversilab System, and to determine the homology of S. aureus isolates from food. Methods： The DNA of 42 S. aureus strains were extrac- ted, and tested with rep- PCR, then homologous analysis was cai＇ried out by Diversilab System. Results： Forty - two strains of S. attreus were divided into 14 groups, the similarity among those strains ranged from 68.9%～ 99.7% , exhibiting no area specificity. Conclusion： Diversilab System offers efficient and excellent discriminato~ power with high reproducibility, so it could be used for typing and tracing source of S. aureus.

关 键 词：Diversilab系统 金黄色葡萄球菌 同源性分析 

分 类 号：R446.5[医药卫生—诊断学]