两种方法测定心肌损伤标志物定量结果的比对分析及联合定性检测的早期诊断价值  

Results comparison of detecting troponin I by POCT and chemiluminescence method and the value of Myo-CK-MB-cTnI combined detection in early diagnosis

作　　者：黄玲玲[1] 李祥云[1] 潘峰[1] 刘玉华[1] 楼洪萍[1] 

机构地区：[1]杭州师范大学附属医院检验科,杭州310015

出　　处：《中国卫生检验杂志》2013年第2期392-394,共3页Chinese Journal of Health Laboratory Technology

基　　金：杭州市医药卫生科技计划项目(20100633B17)

摘　　要：目的:对比评价POCT法和化学发光法定量检测cTnI的检测效能和可靠性,并探讨POCT法Myo、CK-MB和cTnI三项联合定性检测对AMI患者早期诊断的价值。方法:收集60例患者,采用POCT法和化学发光法对cTnI进行测定,对结果进行比较和分析;并对42例早期入院病人在不同时点进行三项联合检测,比较敏感性。结果:在cTnI阴性和高浓度时,仅化学发光法能检测;在低和中浓度时两种方法之间没有统计学差异(P>0.05),但相关性很强;三项联合定性检测中Myo升高时间最快,敏感性高。结论:POCT法快速简便,适于快速诊断;化学发光法灵敏度高,特异性和准确性好,适于确诊和监测。Objective：To evaluate and compare the clinical performance and reliability of POCT and chendh,mi- nescence method in the determination of troponin I, and to study the value of Myo - CK - MB - cTnI combined de- tection in early diagnosis for acute myocardial infarction （AMI） by POCT. Methods： Troponin I levels were detec- ted with both chemiluminescence and POCT methods in 60 patients. The results were compared and analyzed. And 42 cases of early stage patients were selected to carry out Myo - CK - MB - cTNI combined detection at diffcr~~t time, then to compare the sensitivity. Results： When the troponin I levels were ohviously low and high, results shown by two methods were significantly different, and only chemiluminescence could used for detection. While at low and mediate levels, troponin 1 showed no statistical difference between the two methods （P 〉 0.05） , bu! high linear correlation. Meanwhile, Myo showed the quickest rise and high sensitivity in combined detection. Conclu- sion： Being quick and simple, POCT is suitable for initial screening. Chemiluminescence is beneficial for the diag- nosis and monitoring because of its high sensitivity and good accuracy.

关 键 词：即时检验法(POCT) 电化学发光法 肌钙蛋白I(cTnI) 肌红蛋白(Myo) 肌酸激酶同工酶(CK-MB) 急性心肌梗死(AMI) 

分 类 号：R54[医药卫生—心血管疾病]