麻疯树花粉萌发条件分析及percoll分离体系的建立  

Analysis on the conditions of the Jatropha curcas L.pollen germination and the establishment of percoll separation system

作　　者：刘波洋[1] 吴军[1] 唐琳[1] 李美玉[1] 李颖[1] 凌云[1] 陈放[1] 

机构地区：[1]四川大学生命科学学院,成都610064

出　　处：《四川大学学报（自然科学版）》2013年第1期165-170,共6页Journal of Sichuan University(Natural Science Edition)

基　　金：国家科技支撑计划(2011BAD22B00);四川省应用基础研究(2011JY0005)

摘　　要：为了研究离体条件下,不同培养条件对麻疯树花粉萌发的影响,取盛花期麻疯树花粉,分别在不同琼脂浓度、蔗糖浓度、温度、不同时间段下,检测麻疯树花粉萌发情况.结果表明,麻疯树花粉更易在3%的琼脂浓度、10%的蔗糖浓度、25～30℃的条件下萌发,并以此建立了优良的麻疯树花粉萌发培养基(3%琼脂+10%蔗糖+无机液体培养基).进一步研究显示,一天中的不同时间,麻疯树花粉活力不同,具有两个活力高峰期,分别为上午10～11点和下午16～17点.同时,采用Percoll密度梯度离心法对麻疯树花粉进行分离和筛选,结果表明,该方法可以有效的分离麻疯树花粉,不同层花粉活力不同,其中第六层花粉萌发率可高达90%以上,而前五层花粉萌发率均在30%以下.To study the impacts of different culture conditions on pollen germination in vitro, we detected the germination of Jatropha curcas L. pollen at the full-blossom stage subject to different agar and su- crose concentrations, temperature, and time points. The results showed that it was easier for Jatropha curcas L. pollen to germinate in 3% agar, 10% sucrose, and 25~30℃, and the pollen vitality changed along the time with two crest-time,10---11 a. m and 16~17 p.m. Moreover, Jatropha curcas L. pollen was separated and screened through Percoll density gradient centrifugation with high vitality. The ger- mination rate of layer 6 was over 90%, while the others were all less than 30%.

关 键 词：麻疯树 花粉 离体培养 Percoll密度梯度离心 

分 类 号：Q945[生物学—植物学]