野生通江百合高频率再生植株的研究  

High efficiency of plant regeneration from wild lily(Lilium sargentiae Wilson)

作　　者：易欣[1] 周颂东[1] 何兴金[1] 

机构地区：[1]四川大学生命科学学院生物资源与生态环境教育部重点实验室,成都610065

出　　处：《四川大学学报（自然科学版）》2013年第1期193-198,共6页Journal of Sichuan University(Natural Science Edition)

基　　金：国家自然科学基金(31270241;31100161;31070166);教育部博士点基金(20090181110064);科技部基础性研究专项重点项目(2009FY110100)

摘　　要：本文报道了用野生通江百合的种子萌发的无菌苗,切取叶片、叶柄、茎段基部小鳞茎为外植体进行组织培养研究.结果表明:种子在预处理去翅后,经75%(v/v)酒精浸泡杀菌30s,0.1%(v/v)氯化汞消毒5min,灭菌效果最好;愈伤组织诱导的最适培养基为MS+6-BA1.0mg/L+NAA 1.0mg/L+蔗糖30g/L;培养基MS+6-BA2.5mg/L+NAA 0.1mg/L+蔗糖30g/L诱导不定芽及增殖效果最好,平均繁殖倍数9.38,增殖率100.0%;培养基MS+IBA1.00mg/L+蔗糖30g/L最适合用于诱导通江百合不定芽生根,生根率可达93.3%;炼苗后移栽成活率95%以上.The leaves, petiole and bulbs of wild lily （Lilium sargentiae Wilson） seedling were used as explants in tissue culture. The results showed that the treatment of 0.1 % HgCl2 corrosive sublimate for 5 min in after removing the seed wings and soaking in 75%alcohol for 30 s had the best sterilization effect. MS＋6-BA 1.0 mg/L＋NAA 1.0 mg/L＋sucrose 30 g/L was the best medium to induce callus. The optimal medium for inducing shoots and proliferation was MS^6-BA 2.5 mg/L＋NAA 0. 1mg/L＋sucrose 30 g/L. Its Ave. multiples of propagation was 9.38 while proliferation rate reached 100~. MS ＋IBA1.00mg/L＋sucrose 30 g/L was suitable for the shoots rooting. 93.3% plantlets with roots were obtained and the survival rate of transplanting reached 95%.

关 键 词：通江百合 种子 组织培养 

分 类 号：Q945.5[生物学—植物学] S682.2[农业科学—观赏园艺]