高淀粉专用型甘薯品种悬浮体系的建立及植株再生  

Establish of Cell Suspension Culture and Plant Regeneration of Sweet Potato Varieties with Specialized Uses of High Starch

作　　者：吴洁[1] 郑雪莲[2] 屈会娟[1] 李明[1] 阎文昭[1] 

机构地区：[1]四川省农业科学院生物技术核技术研究所,四川成都610066 [2]电子科技大学生命科学与技术学院,四川成都610054

出　　处：《西南农业学报》2013年第1期52-56,共5页Southwest China Journal of Agricultural Sciences

基　　金：四川省应用基础项目(2009JY0100);四川省财政基因工程专项(2011JYGC01-005);四川省农作物育种攻关项目

摘　　要：利用目前四川省主推的6个高淀粉甘薯品种进行悬浮培养及有效植株再生的研究,对以MS为基础的10种胚性愈伤培养基进行分析,结果表明,茎尖分生组织培养基在添加1.8 mg/L 2,4-D、30 mg/L蔗糖的MS培养基上诱导的胚性愈伤组织最多。胚性愈伤组织在添加1.8 mg/L 2,4-D、30 mg/L蔗糖的MS培养基上进行震荡培养建立悬浮细胞系,悬浮培养40周后,进行植株再生培养,比较了3种以MS为基础的再生培养基,将直径大于1cm的大细胞团放在添加1.5 mg/L ABA、30 mg/L蔗糖的MS上获得再生植株最多,再生率为96%。In this paper, 6 varieties of high starch sweet potato, which was mainly popularized variety in Sichuan province, were studied in call suspension culture and effective plant regeneration, and 10 types of embryonic callus medium on the basis of MS were compared and ana- lyzed, the result showed that the tip meristem culture could induce the most embryogenic callus on MS medium with 1.8 mg/L 2,4-D ＋ 30 mg/L sucrose. Embryonic callus cultured with shaking on MS medium with 1.8 mg/L 2,4-D ＋30 rag/L sucrose could establish suspension cell system after 40 weeks suspension culture, then was cultured for regeneration. 3 more types regeneration medium on the basis of MS were compared, and I cm （in diameter） multicellular clusters could acquire the most regeneration plants on the MS medium with 1.5 rag/L ABA ＋30 mg/L sucrose, of which regeneration rate was 96 %.

关 键 词：高淀粉 细胞悬浮培养 细胞团 植株再生 

分 类 号：S531[农业科学—作物学]