大麦种子萌发期对干旱胁迫的生理响应及其抗旱性评价  

Physiological Response of Barley to Drought Stress during Seed Germination Period and Drought Resistance Evaluation

作　　者：鞠乐[1] 齐军仓[1] 成禄艳[1] 赵佳[1] 廖永兵[1] 

机构地区：[1]新疆石河子大学农学院,新疆石河子832003

出　　处：《西南农业学报》2013年第1期93-98,共6页Southwest China Journal of Agricultural Sciences

基　　金：农业部现代农业产业技术体系建设专项资金(CARS-05);兵团动植物新品种选育科技攻关专项(2011BA002);石河子大学科学技术研究发展计划"动植物育种专项"(gxjs2011-yz05)

摘　　要：以4个抗旱性差异的大麦品种为材料,用浓度水平为0%、5%、10%、15%、20%(w/v)PEG8000溶液模拟干旱胁迫处理,研究丙二醛、脯氨酸、可溶性糖、可溶性蛋白、过氧化氢酶、过氧化物酶、超氧化物歧化酶等生理生化指标对干旱胁迫的响应及其与抗旱性之间的相关性,进行抗旱性评价与抗旱性鉴定指标的筛选。结果表明:随着干旱胁迫程度的增加,超氧化物歧化酶活性、丙二醛、脯氨酸、可溶性糖、可溶性蛋白含量变化均呈显著的上升趋势,过氧化氢酶和过氧化物酶活性的变化基本呈先上升后下降的趋势。20%PEG8000是较适宜的抗旱性鉴定浓度,抗旱性强弱排序为Z037P017Q-1>Z182U038V>Z039P046Q>Z027S078T。过氧化氢酶、过氧化物酶和超氧化物歧化酶活性可作为大麦种质资源种子萌发期抗旱性鉴定的指标。The responses of malondialdehyde, prelinc, soluble sugar, soluble protein, catalase, peroxidase and superoxide dismutase to drought stress and the drought resistance of 4 barley varieties were studied by utilizing4 barley varieties with different drought resistance trea- ted with different PEGS000 concentrations （0 %, 5 %, 10 %, 15 % and 20 % ） as drought stress,and the indices of drought resistance were screened in this paper. The results showed that the activity of superoxide dismutase and the contents of malondialdehyde, preline, solu- ble sugar, soluble protein increased significantly with the increase of drought stress; the activities of catalase and peroxidase firstly increased, then decreased; the suitable PEGS000 concentration for drought resistance identification was 20 % ; the pecking order of drought resistance was Z037P017Q-1, Z182U038V, Z039P046Q and Z027S078T; the activities of catalase, perexidase and superexide dismutase could be used as an effective identification indices for drought resistance during seed germination period.

关 键 词：大麦 萌发期 干旱胁迫 抗旱性 生理生化指标 

分 类 号：S512.2[农业科学—作物学]