ptc-miR213的人工microRNA植物表达载体的构建及遗传转化  

Construction of Artificial miRNA of ptc-miR213 Expression Vector and Transformation into Arabidopsis thaliana

作　　者：谢丽华[1] 蒋晶[1] 刘明英[1] 乔桂荣[1] 邱文敏[1] 杨惠琴[1] 卓仁英[1] 

机构地区：[1]中国林业科学研究院亚热带林业研究所,浙江富阳311400

出　　处：《林业科学研究》2013年第1期29-33,共5页Forest Research

基　　金：浙江省科技厅重大专项"重要生态经济树种转基因平台建立及耐盐转基因研究"(2010C12010);浙江省自然科学基金项目(30972340)

摘　　要：ptc-miR213是在构建盐胁迫条件下青杨microRNA文库中发现的,预测其靶基因为MYB4、PP2C、蛋白激酶等,其中MYB家族与植物的耐盐性密切相关。为了探索ptc-miR213在植物盐碱胁迫应答方面的作用,实验构建了植物表达载体pCAM2300-ami213,经根癌农杆菌EHA105介导转化拟南芥,成功获得转基因植株。RT-PCR分析表明amiR213在转基因拟南芥中能够超量表达。本研究为分析ptc-miR213及其靶基因参与植物盐碱胁迫应答奠定了基础。The ptc-miR213 was identified from Populus cathayana by microRNA library sequence. It has been con- firmed that the ptc-miR213 is induced by salt stress. The putative targets are MYB4, PP2C and protein kinase, in which MYB may play an important role in response to salt stress. To investigate the function of ptc-miR213, the ar- tificial miRNA213 expression vector was constructed and transformed into Arabidopsis thaliana. The transgenic plants were confirmed by PCR and RT-PCR. The results showed that amiRNA transcript level was markedly im- proved in transgenic plant compared with non-transgenic plants.

关 键 词：amiRNA 拟南芥 花序浸染 盐胁迫 

分 类 号：S718.46[农业科学—林学]