卫分证、气分证、营分证Th1/Th2漂移变化规律研究  

Study on the law of drifting of Th1/Th2 in wei phase syndrome,qi phase syndrome,ying phase syndrome of acute suppurative tonsillitis

作　　者：刘旺华[1] 刘建新[2] 周小青[1] 谢静[3] 

机构地区：[1]湖南中医药大学中医诊断学重点学科,中医诊断学精品课程,中医诊断学教学团队,中医诊断学实验室,病理生理实验室,中医病症实验室 [2]湖南中医药大学中医学院 [3]湖南中医药大学第一附属医院,湖南长沙410007

出　　处：《湖南中医药大学学报》2013年第1期118-120,共3页Journal of Hunan University of Chinese Medicine

基　　金：湖南省教育厅科研项目(08C621;10A093);湖南省高校创新平台开放基金项目(101C047)

摘　　要：目的探讨卫分证、气分证、营分证Th1/Th2漂移变化规律,为卫气营血辨证提供客观的实验指标。方法将50例患者按中医辨证分为卫分证组20例,气分证组15例,营分证组15例。另选择体检健康者l6例为健康对照组。用ELISA法检测不同阶段患者血清Th1和Th2细胞因子白细胞介素(IL-2)、肿瘤坏死因子(TNF-β)、γ-干扰素(IFN-γ)、IL-4、IL-5、IL-6水平的变化。结果与正常组比较,卫分证组、气分证组、营分证组IL-2、IFN-γ水平明显升高(P<0.05,P<0.01),气分证组、营分证组TNF-β、IL-4、IL-5、IL-6、IL-10水平显著上升(P<0.01);与卫分证组比较,气分证组IL-2、TNF-β、IFN-γ、IL-4、IL-5、IL-6、IL-10水平显著上升(P<0.05,P<0.01),营分证组TNF-β、IL-4、IL-5、IL-6、IL-10水平显著上升(P<0.01);与气分证组比较,营分证组IL-2、TNF-β、IFN-γ均有下降趋势,IFN-γ下降差异有统计学意义(P<0.01),而IL-4、IL-6、IL-10继续上升(P<0.05,P<0.01)。结论从卫分证到气分证再到营分证病理发展与Th1/Th2漂移密切相关,IL-2、TNF-β、IFN-γ、IL-4、IL-5、IL-6、IL-10的动态变化可作为卫气营血辨证的客观指标之一。Objective To discuss the law of phase syndrome, ying phase syndrome of acute drifting of Th1/Th2 in wei phase syndrome, qi suppurative tonsillitis. Methods 50 patients were divided into wei phase syndrome group （20）, qi phase syndrome group （15）, ying phase syn- drome group（15） according to differentiating symptoms of TCM, and 16 healthy people were select- ed as the normal control. The expressions of IL-2, TNF-β, IFN-γ, IL-4, IL-5, IL-6 and IL-10 were detected by ELISA. Results Compared with normal group, the levels of IL-2 and IFN-γ of wei phase syndrome group, qi phase syndrome, ying phase syndrome group increased significantly （P〈0.05 or P〈0.01）, and the contain of TNF-15, IL-4, IL-5, IL-6 and IL-10 of qi phase syndrome, ying phase syndrome group improved markedly （P〈0.01）. Compared with wei phase syndrome group, the levels of IL-β, TNF-~, IFN-γ IL-4, IL-5, IL-6 and IL-10 of qi phase syndrome increased instinctly （P〈0.05 or P〈0.01）, and the levels of TNF-γ, IL-4, IL-5, IL-6 and IL-10 in- creased markedly in ying phase syndrome group （P〈0.01）. Compared with qi phase syndrome group,the levels of IL-2, TNF-13 and IFN-γ were tending downwards, and the level of IFN-γ decreased instinctly in ying phase syndrome group （P〈0.01）, while the levels of IL-4, IL-6 and IL-10 in- creased constantly. Conclusion Pathologic developing process from wei phase syndrome to qi phase syndrome and then to ying phase syndrome is correlated closely to the drifting of Thl/Th2 and the dynamic changes of IL-2, TNF-β, IFN-γ IL-4, IL-5, IL-6 and IL-10 can be used as one of the objective indicators in differentiation of Wei-Qi-Ying-Xue.

关 键 词：卫气营血辨证 卫分证 气分证 营分证 TH1 Th2漂移 干扰素 肿瘤坏死因子 白细胞介素 

分 类 号：R241.8[医药卫生—中医诊断学]