电穿孔转染重组杆状病毒到昆虫细胞的研究  

The Research of Electroporation Transfect Restructured Baculovirus Genome into Insects Cells

作　　者：陈卫东[1] 杨靖佳[1] 井申荣[1] 曾韦锟[1] 黄芬[1] 

机构地区：[1]昆明理工大学生命科学与技术学院,中国云南昆明650500

出　　处：《生命科学研究》2013年第1期5-10,共6页Life Science Research

基　　金：国家自然科学基金资助项目(30872398;31160193);云南省应用基础研究项目(2007C032M)

摘　　要：目前转染细胞时常用阳离子脂质体如Cellfectin,这种方法成本高,效果不稳定,而用电穿孔转染外源基因到昆虫细胞的文献报道极为少见.构建重组杆状病毒基因组GFP/BAC,用电穿孔法和脂质体法转染到昆虫细胞Sf9中,比较两种方法的转染效率.研究发现,两者转染效率分别为86.14%和86.53%.两种转染方法的子代重组杆状病毒能够继续转染Sf9细胞,荧光强度无明显差异.电穿孔转染效率与脂质体转染接近,但电穿孔法操作简便,周期较短,成本很低.The basic ion liposome, such as Cellfectin, is usually Used to transfect cell at present. The short- age of this method is expensive and instable. While the;literature report that transfect exogenous gene into insects cells by electroporation is very rare. Construct the rbstructured baculovirus genome GFP/BAC and transfect into the insects cells Sf9 by electroporation and： liposome Cellfectin, then compare the transfection efficiency of these two methods. The result show that the transfection efficiency of the two methods respec- tively is 86.14% and 86.53%, and the filial generation restructured baculovirus continue transfect Sf9 cell and the fluorescence intensity of these two methods was not obviously distinct. The transfection efficiency of electroporation is compared with liposome Cellfectin, while the advantages of electroporation is simple opera- tion, short cycle and low cost.

关 键 词：电穿孔 转染效率 重组杆状病毒 昆虫细胞 

分 类 号：Q31[生物学—遗传学]