用细胞膜脂脂肪酸成分分析法筛选抗寒巨桉种源  

Selection of the Cold resistant Species Provenances of Eucalyptus grandis by Using the Method of Composition Analysis of Fatty Acids of Cell Membrane

作　　者：孙汉洲[1] 赵芳[1] 李志辉[1] 

机构地区：[1]中南林学院基础课部,中国湖南株洲412006

出　　处：《中南林学院学报》2000年第3期59-62,共4页Journal of Central South Forestry University

基　　金：国家重点科技攻关项目!"桉树纸浆用材树种良种选育及培育技术研究"( 96-0 11-0 1-0 3 )的部分内容

摘　　要：为了选育抗寒性好的巨桉品种 ,利用气相色谱法分析了巨桉 12个种源的 80个家系样品的细胞膜脂肪酸组成和成分 .采用 KOH-CH3OH-BF3酯化、硅胶脱色和程序升温的方法 ,将细胞膜中的主要脂肪酸——棕榈酸 ( 16∶ 0 )、棕榈油酸 ( 16∶ 1)、硬脂酸( 18∶ 0 )、油酸 ( 18∶ 1)、亚油酸 ( 18∶ 2 )和亚麻酸 ( 18∶ 3 )完全分离 .运用归一化方法得出了上述各种脂肪酸含量 .通过对 IUFA分析 ,筛选出了抗寒性好的 18,14,3 9,3 8,2 6,3 5 ,3 6,3 7八个家系品种和抗寒性差的 2 ,80 ,5 8,5 1,70 ,68,62 ,67,60九个家系品种 。To select good cold resistant provenance of Eucalyptus grandis ,the method of gas chromatograpy was used to analyse the composition and content of fatty acids and 80 families belonging to 12 provenance of Eucalyptus grandis .The experiment showed that the main fatty acids in the cell membrane——palmitic acid(16∶0),palmitoleic acid(16∶1),setearic acid(18∶0),oleic acid(18∶1),linoleic acid(18∶2) and eleosetearic acid(18∶3) could be separated completely by using the method of KOH CH 3OH BF 3 esterify ,silica gel decolorition and the programming calefaction.The content of the main acids could be obtained through normalization method.By analyzing the IUFA we have got eight families of good cold resistant provenance such as No.18,No.14,No.39,No.38,No.26,No.35,No.36,No.37 and nine families of poor cold resistant provenance such as No.2,No.80,No.58,No.51,No.70,No.68,No.62,No.67,No.60.The result obtained in this way was correspondent with the practical winter field experiement.

关 键 词：巨桉 抗寒性 种源 细胞膜脂肪酸成分 

分 类 号：S792.39[农业科学—林木遗传育种] S722.33[农业科学—林学]