新建产PHB基因工程菌VG1(pTU14)的碳源代谢  

Carbon metabolism of a novel recombinant Escherichia coli used for poly-β-hydroxybutyrate production

作　　者：于慧敏[1] 尹进[1] 李红旗[1] 杨胜利[2] 沈忠耀[1] 

机构地区：[1]清华大学化学工程系,生物化工研究所北京100084 [2]中国科学院上海生物工程研究中心,上海200233

出　　处：《清华大学学报（自然科学版）》2000年第10期54-56,共3页Journal of Tsinghua University(Science and Technology)

基　　金：国家"九五"科技攻关项目!(96 - C0 3- 0 3- 0 2 );国家自然科学基金重点项目!(2 9834 10 3);国家自然科学基金面上项目!(2 9876 0

摘　　要：为解决聚 β-羟基丁酸酯 (PHB)生产过程中的高成本问题 ,构建了具有高溶氧利用能力及可自动裂解破壁的PHB高产基因工程菌 VG1(p TU14)。对该重组菌的葡萄糖耐受能力、乙酸的辅助同化作用及较廉价的淀粉水解液替代葡萄糖的可行性等进行了研究。该重组菌可以在初糖浓度2 0 0 g/ L 的培养基中良好生长 ;无机氮源乙酸铵可同时提供乙酸作为辅助碳源 ;采用淀粉水解液可以完全替代葡萄糖 ,发酵结束时菌体生长和 PHB积累均提高 ,还原糖利用率也可达到 99%以上。To reduce the high cost of poly β hydroxybutyrate (PHB) production, Vitreoscilla hemoglobin gene (vgb), lytic genes with the S amber mutation of the phage lambda (S RRz) and PHB biosynthetic genes (phbCAB) were introduced into the same cell of E.coli JM105 by cloning. A novel recombinant multi functional strain VG1 (pTU14) was constructed which had high oxygen uptake, inducible autolysis and high PHB accumulation. The novel strain could even grow in broth with a high initial glucose concentration of 200g/L. Ammonium acetate, which was the inorganic nitrogen in the medium, was also a carbon source, co promoting the PHB accumulation in the novel strain. In addition, the less expensive starch hydrolysate could be substituted for glucose, with increased cell growth, PHB accumulation and 99% of the consuming yield of the reduced dextrose in the starch hydrolysate.

关 键 词：聚Β-羟基丁酸酯 基因工程菌 碳源代谢 

分 类 号：Q493[生物学—生理学] Q939.1