人前列腺癌细胞原代培养及纯化培养的体外药敏试验  

Chemosensitivity Testing for Human Prostatic Cancer with Primary Culture Cells and Purified Culture Cells Using the CCK-8 Assay

作　　者：卜强[1] 蒋华[1] 虞小明[1] 

机构地区：[1]南通大学附属丹阳人民医院,江苏丹阳212300

出　　处：《药学与临床研究》2013年第1期13-16,共4页Pharmaceutical and Clinical Research

基　　金：江苏省卫生厅资助项目(No:P200956)

摘　　要：目的:建立人前列腺癌细胞体外药敏实验,指导临床前列腺癌化疗。方法:收集前列腺癌组织标本,进行原代培养及纯化培养,获得前列腺癌细胞。接种96孔板,给予1×PPC多西他赛(DOC)、羟基喜树碱(HCPT)、顺铂(DDP)、依托泊苷(VP-16)、阿霉素(ADM)和丝裂霉素(MMC)处理,采用CCK-8法观察上述化疗药物对前列腺癌细胞的抑制率。结果:前列腺癌细胞对于上述化疗药物的敏感性依次是:DOC>HCPT>VP-16>DDP>ADM>MMC。在本实验条件下前列腺癌细胞原代培养成功率为78.1%(50/64),纯化培养成功率为65.6%(42/64)。原代培养平均耗时(16±4.2)天(n=50),纯化培养平均耗时(53±12.6)天(n=42)。纯化培养的前列腺癌细胞对化疗药物的敏感性高于原代培养的细胞。结论:原代培养前列腺癌细胞药敏实验操作简便,成功率高,耗时短,与纯化培养药敏结果相关性好,可以用于指导前列腺癌的临床治疗。Objectives： To establish a chemosensitivity test for human prostatic cancer cells in vitro and guide clinical prostate cancer chemotherapy. Methods： Prostate cancer tissue samples were collected and primary culture and purified culture were conducted. Then the cells were harvested and inoculated in 96-well plates. Docetaxel （DOC）, hydroxycamptothecin （HCPT）, cisplatin （DDP）, etoposide （VP-16）, adriamycin （ADM） and mitomyein （MMC） were respectively added at the concentration of l xPPC. Then the inhibitory rate of the cells was detected using CCK-8 assay. Results： The order of the inhibitory potency of the drugs on prostatic cancer cells was： DOC〉HCPT〉VP-16〉DDP〉ADM〉MMC. The success rate of primary culture was 78.1% （50/64） and that of purified culture was 65.6% （42/64）. The average time- consuming were 16±4.2 d （n=50） and 53±12.6 d （n=42）, respectively. Purified culture prostate cancer cells were more sensitive to chemotherapeutic drugs than primary culture cells. Conclusions： Primary culture cells for prostate cancer chemosensitivity tests are simple and less time-consuming. As it is well con＇elated with the results of purified culture cells, it can be used to guide clinical chemotherapy of prostate cancer.

关 键 词：前列腺癌 原代培养 纯化培养 药敏试验 

分 类 号：R965[医药卫生—药理学] R737.25[医药卫生—药学]