硫酸氨基葡萄糖胶囊对兔膝关节软骨细胞凋亡基因Bcl-2与Bax表达的影响  

Effects of Glucosamine Sulfate Capsules on Cartilage Cell Apoptosis in Rabbit's Knee Joints

作　　者：林宗汉[1] 郑铁牛[1] 黎强[2] 覃学流[1] 容向宾[1] 龙飞攀[1] 梁庆华[1] 王永乐[1] 

机构地区：[1]广西中医药大学附属瑞康医院骨科,南宁530011 [2]广西中医药大学,南宁530001

出　　处：《药学与临床研究》2013年第1期25-28,共4页Pharmaceutical and Clinical Research

摘　　要：目的:探讨硫酸氨基葡萄糖胶囊延缓软骨组织退变的可能作用机制。方法:采用30只新西兰兔,随机分为药物组、模型组和正常组各10只。前2组通过手术造成右膝关节失稳型动物模型,造模一周始驱赶全部兔子行走,连续4周;药物组并连续给药8周。造模后12周末处死所有动物,取右膝关节内侧胫骨平台软骨组织,采用原位末端标记法检测软骨细胞凋亡率,免疫组化法检测Bcl-2与Bax表达,数据经统计学处理。结果:模型组软骨组织退变明显,细胞凋亡率30.19%±3.08%,Bcl-2表达8.02%±2.09%,Bax表达26.69%±2.78%;药物组软骨组织退变较轻,细胞凋亡率17.01%±1.61%;Bcl-2表达16.34%±2.26%,Bax表达7.19%±1.71%;组间数据比较均有统计学意义。结论:硫酸氨基葡萄糖胶囊可上调兔膝关节软骨细胞凋亡基因Bcl-2的表达,下调Bax表达,减少软骨细胞凋亡,这可能是延缓软骨组织退变的作用机制之一。Objective： To explore the influence and curing on apoptosis of cartilage cells in rabbit＇s knee joints. Methods mechanism of glucosamine gulfate capsules A total of 30 New Zealand rabbits were divided randomly into three groups： drug group, control group and normal group, 10 for each group. Rabbits in the drug and control groups were operated to form knee joint instability, a week later, each rabbit was forced to walk every day for 4 weeks. Glucosamine gulfate capsules were given to the rabbits of drug group for 8 weeks. At the end, the animals were sacrificed and arthrodial cartilages of right tibial plateau were taken to detect the apoptosis rate by in situ end labeling and detect Bcl-2 and Bax expression by immunohistochemistry. Data were statistically processed. Results： The tissue cataplasis of the control group was clear. The rate of apoptosis of the control group was 30.19%±3.08%, the Bcl-2 expression was 8.02%±2.09% and the Bax expression was 26.69%±2.78%. The rate of apoptosis of the drug group was 17.01%±1.61%, the Bcl-2 expression was 16.34%±2.26% and the Bax expression was 7.19%±1.71%. There were significant differences between each two groups. Conclusion： Glucosamine sulfate capsules can obviously up-regulate the apoptosis gene Bcl-2 expression and down-regulate Bax expression, and degrade the apoptosis rate of cartilage cells in rabbit＇s knee joints. A possibility mechanism of glucosamine sulfate capsules is to delay articular cartilage tissue degeneration.

关 键 词：氨基葡萄糖 兔 膝关节 凋亡基因 

分 类 号：R965.1[医药卫生—药理学]