CD4^+ CD25^+ Foxp3^+调节性T细胞在麻风病中的水平和意义  

The level and significance of CD4^+ CD25^+ Foxp3^+ regulation T cells in leprosy

作　　者：李彩霞[1] 徐元品 邹子宏[3] 周晓鸿[4] 

机构地区：[1]云南省昆明市延安医院皮肤科,昆明650051 [2]云南省楚雄州疾病预防控制中心,楚雄651200 [3]云南省昆明市疾病预防控制中心,昆明650011 [4]昆明医科大学第二附属医院皮肤科/风湿免疫科,昆明650022

出　　处：《中国免疫学杂志》2013年第2期158-160,共3页Chinese Journal of Immunology

基　　金：云南省自然科学基金项目(2009CD174)

摘　　要：目的:探讨CD4+CD25+Foxp3+调节性T细胞(Treg)在麻风病发病中的作用。方法:采用流式细胞仪检测51例现症麻风患者,5例发生Ⅱ型麻风反应(ENL)的患者,26例治愈麻风患者及56例正常体检者外周血CD4+CD25+Foxp3+调节性T细胞(Treg)占CD4+的百分比。结果:现症组、治愈组、Ⅱ型麻风反应(ENL)组及正常对照组外周血Treg的水平分别为:(17.626±8.197 7)%、(38.442±4.761 8)%、(6.380±1.548 2)%和(9.998±1.706 2)%。治愈组与现症组麻风患者的Treg高于正常对照组,并且治愈组的Treg比现症组高(P<0.05),ENL组的Treg比正常对照组低(P<0.05);在现症麻风病人中,少菌型患者(PB)及多菌型患者(MB)Treg的百分比分别为:(29.629±7.999 5)%和(15.709±6.480 9)%,均高于正常对照组(9.998±1.706 2)%(P<0.05),并且少菌型患者(PB)的Treg高于多菌型患者(MB)(P<0.05)。结论:治愈组与现症组麻风患者的Treg均高于正常对照组,发生Ⅱ型麻风反患者(ENL组)的Treg比正常对照组低,Treg可能打破了外周免疫耐受,参与了麻风病的发生发展。Objective：To detect the expression and significance of CIM＋ CD25 ＋ Foxp3 ＋ regulation T cells in leprosy. Meth- ods：The leprosy patients were 51, the cured leprosy people were 26, the ENL were 5, and the normal persons were 56. We used the flow eytometry to test the Tregs in four groups above mentioned. And use the SPSS 19.0 to analysis the results. Results ：The percentage of Tregs in leprosy patients, cured people, ENL patients and normal persons were （ 17. 626 ± 8. 1977 ） %, （38. 442 ±4.7618 ） %, （6. 380 ± 1. 5482）% and （9.998 ± 1. 7062）%. The Tregs in cured people and leprosy were higher than normal and the Tregs in cured people were higher than leprosy patients, （P 〈 0.05 ） ; The Tregs in ENL were lower than normal;Among leprosy patients （P 〈 0.05 ）, the Tregs in PB was higher than MB, （P 〈0.05 ）. Conclusion：Tregs may break the immune tolerance and related to the leprosy.

关 键 词：麻风病 CD4+CD25+Foxp3+ TREG 流式细胞仪 

分 类 号：R755.01[医药卫生—皮肤病学与性病学]