Lactobacillus casei胞外多糖对BALB/c小鼠肠相关淋巴细胞调控的初步研究  

Effect of exopolysaccharide from Lactobacillus casei on murine gut-associated lymphocytes

作　　者：包鹏[1] 唐彦君[2] 刘宁 

机构地区：[1]东北农业大学食品学院乳品科学教育部重点实验室,哈尔滨150030 [2]黑龙江八一农垦大学食品学院,大庆163319 [3]国家乳业工程技术研究中心,哈尔滨150028

出　　处：《中国免疫学杂志》2013年第2期182-186,共5页Chinese Journal of Immunology

基　　金：教育部创新团队基金(IRT-0959-202);黑龙江省研究生创新科研项目(YJSCX2012-043HLJ);哈尔滨市科技创新人才研究专项资金项目(2011RFXXN042)资助

摘　　要：目的:探讨干酪乳杆菌胞外多糖(EPS)对BALB/c小鼠小肠相关淋巴细胞增殖及细胞因子TNF-α、IL-17及IFN-γ分泌的影响。方法:Percoll不连续密度梯度分离法从小鼠小肠中分离上皮内淋巴细胞(IEL)、固有层淋巴细胞(LPL)、派氏集合淋巴结淋巴细胞(PPL)和肠系膜淋巴结淋巴细胞(MLNL)。实验组分别加入10、25、50、100和200μg/ml浓度的干酪乳杆菌胞外多糖。水溶性四氮唑(WST-1)法检测淋巴细胞增殖;ELISA法测定细胞上清液中细胞因子TNF-α、IL-17及IFN-γ的含量。结果:EPS能够一定程度地促进IEL、LPL、PPL的增殖,且均在100μg/ml浓度时达到最大值,在50～200μg/ml浓度范围内促进MLNL增殖呈剂量依赖性,EPS能够使IEL、LPL和PPL上清液中TNF-α含量降低,且在50～200μg/ml浓度时差异极显著(P<0.01),但对MLNL分泌TNF-α无明显抑制作用,在200μg/ml时有显著提高(P<0.01);EPS可以使IEL、LPL、PPL及MLNL上清液中IL-17含量升高;EPS对IEL、LPL、PPL及MLNL上清液中IFN-γ无明显影响。结论:EPS可以促进小鼠小肠相关淋巴细胞的增殖,并能够调控细胞因子TNF-α及IL-17的分泌水平。Objective：To study the effect of exopolysaccharide （EPS） from LactobaciUus casei on murine gut-associated lym- phocytes proliferation and production of TNF-α, IL-17 and IFN-7. nethods：intraepithelial lymphocytes （IEL）, lamina propria lym- phocytes （LPL）, Peyer＇s Patches lymphocytes （PPL） and mesenteric lymph nodes lymphocytes （MLNL） were isolated from small in- testine of mice by using Percoll discontinuous density gradient centrifugation. WST-1 assay was used to determine the proliferation of lymphocyte. The secretion of TNF-α, IL-17, IFN-7 in cell culture supernatant was assayed by ELISA. Results ：EPS could induce IEL, LPL, and PPL proliferation, and increased the proliferation obviously at 100 μg/ml, EPS could promote the proliferation MNLN at 50- 200 Ixg/ml, which was dose-dependent. EPS demonstrated inhibitory activity to the secretion of TNF-α in IEL, LPL and PPL and there were significant differences in the control group at 50-200μg/ml. EPS could not depress the secretion of TNF-α in MLNL, and in- creased the secretion of TNF-α obviously at 200 μg/ml EPS demonstrated facilitative activity to the secretion of IL-17 in IEL, LPL, PPL and MLNL EPS could not influence the secretion of IFN-r. Conclusion：EPS can induce the proliferation of gut-associated lymph- oid tissues＇ lymphocytes, and regulate TNF-α, IL-17 secretion.

关 键 词：干酪乳杆菌胞外多糖 肠相关淋巴组织 淋巴细胞 细胞因子 调节 

分 类 号：R392.4[医药卫生—免疫学]