复方蒲芩胶囊含量测定方法的改进  

Improvement of Determination of Fufang Puqin Capsules

作　　者：戚宝婵[1] 孙苓苓[1] 李子[1] 

机构地区：[1]辽宁省食品药品检验所,沈阳110023

出　　处：《中国药品标准》2013年第1期33-35,共3页Drug Standards of China

摘　　要：目的:改进复方蒲芩胶囊的含量测定方法。方法:采用HPLC法同时测定复方蒲芩胶囊中黄芩苷和盐酸小檗碱的含量。色谱柱为Diamonsil C18(4.6 mm×200 mm,5μm),以乙腈-0.05 mol.L-1磷酸二氢钠溶液(29∶71)(用磷酸调节pH值至3.0)为流动相,检测波长为280 nm。结果:黄芩苷和盐酸小檗碱的线性范围分别为0.056 57～0.848 6μg(r=0.999 9)和0.254 9～3.823 5μg(r=0.999 9);平均回收率分别为98.0%(RSD=2.2%),97.9%(RSD=0.97%)。结论:方法简便、准确,重复性好,可用于复方蒲芩胶囊质量控制。Objective： To improve the method for determination of Fufang Puqin Capsules. Methods： To establish an HPLC method for simultaneous determination of baicalin and berberine hydroehloride in Fufang Pulqin Capsules. The chromatographic separation was achieved on a Diamonsil CIs （4. 6 mm × 200 mm, 5 μm） column with acetonitrile-0.05 mol · L -1 sodium dehydrogenate phosphate（29 ：71 ） （adjusted to pH 3.0 with phosphoric acid）as mobile phase and the detective wavelength was set at 280 nm. Results： The calibration carves of baicalin and berberine hydrochloride were linear in the ranges of 0. 056 57 - 0. 848 6 μg （ r = 0.999 9 ） , 0. 254 9 - 3. 823 5·g （r =0. 999 9） respectively. The average recoveries were 98. 0% with RSD 2. 2% , 97. 9% with RSD 0. 97% respectively. Conclusion： The method is simple, accurate and reproducible for quality control of Fufang Pulqin capsules.

关 键 词：复方蒲芩胶囊 黄芩苷 盐酸小檗碱 高效液相色谱法 

分 类 号：R912.2[医药卫生—药学]