清喉咽合剂中连翘苷不同定量方法对比分析  

Comparative Analysis of Different Quantitative Methods for Forsythin in Qinghouyan Mixture

作　　者：张振巍[1] 张娜娜 白丹丹[1] 张建民[1] 

机构地区：[1]中国人民解放军第155医院,河南开封475000 [2]开封市中医院,河南开封475000

出　　处：《中国中医药信息杂志》2013年第3期65-66,83,共3页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的对比不同分析方法对清喉咽合剂中连翘苷含量的影响。方法采用高效液相色谱法(HPLC)和三波长-分光光度计对清喉咽合剂中连翘苷进行含量测定。结果连翘苷在0.025 1～0.125 6 mg/mL范围内,分别在波长278.5、248.0、272.4 nm处测定吸收度时,ΔA与浓度C之间呈现较好的线性关系,回归方程为ΔA=89.705C-0.041,r=0.999 7;平均加样回收率为97.48%,RSD=1.88%。三波长测定法与HPLC测定结果比较,差异无统计学意义。结论三波长测定法对供试品制备要求简单,操作简便易行,试验的重复性好,效率高,可以作为该制剂组分含量测定方法。Objective To comparatively analyze the different methods on effect of forsythin content in Qinghouyan Mixture. Methods The content of forsythin in Qinghouyan Mixture was quantitatively analyzed by three-wavelength spectrophotometry and HPLC method. Results The absorbance was measured at wavelength of 278.5 nm, 248.0 nm and 272.4 nm. The regression equation of the proposed method was obtained as follows. AA----89.705C--0.041, r=0.9997. The linear relationship was good in the range of 0.0251--0.1256 mg/mL. The average recovery rate was 97.48% and RSD was 1.88%. The determination results had no statistical difference with that of HPLC. Conclusion The repeatability of experiment is good and the method is simple, fast and efficient. It can be applied to the determination of the preparation.

关 键 词：三波长-分光光度法 高效液相色谱法 清喉咽合剂 连翘苷 

分 类 号：R284.1[医药卫生—中药学]