凝血酶敏感蛋白-1在液压冲击损伤体外培养大鼠海马神经细胞中的表达及意义  

Expression and significance of thrombospondin-1 in rats′ hippocampal neurons cultured in vitro by fluid percussion injury

作　　者：杨术旺[1] 梁林[1] 战丽[1] 舒清明[2] 秦晓静[1] 张永亮[1] 董化江[1] 

机构地区：[1]中国人民武装警察部队后勤学院训练部研究生处,天津300162 [2]中国人民武装警察部队总医院病理科,北京100039

出　　处：《新乡医学院学报》2013年第2期81-85,共5页Journal of Xinxiang Medical University

基　　金：国家自然科学基金资助项目(编号:30820004)

摘　　要：目的探讨凝血酶敏感蛋白-1(TSP-1)在液压冲击损伤大鼠体外培养海马神经细胞中的表达。方法建立体外原代培养神经细胞液压冲击损伤模型,采用甲苯胺蓝染色观察神经细胞损伤程度,免疫细胞化学染色和Western blot技术检测神经细胞中TSP-1的表达水平。结果液压冲击后,甲苯胺蓝染色显示,损伤组部分神经细胞胞体浓缩,细胞核固缩,呈深蓝色,多为坏死型损伤细胞。免疫细胞化学染色显示,对照组神经细胞内有一定量的TSP-1表达,损伤组神经细胞内TSP-1表达增多,阳性细胞数目增多。Western blot结果显示,对照组和损伤组光密度值分别为0.937±0.194与2.318±0.495,损伤组神经细胞TSP-1蛋白表达显著强于对照组(P<0.01)。结论体外培养大鼠海马神经细胞中TSP-1高表达可能在液压冲击损伤过程中起重要作用。Objective To investigate the expression of thrombospondin-1 （TSP-1） in hippocampal neurons of rats cultured in vitro by fluid percussion injury. Methods The primary cultural neuron models of fluid percussion injury were estab- lished. The degree of neurons injury was observed by toluidine blue stain. The expression of TSP-1 in neurons was detected by Western blot and immunocytochemical stain. Results After fluid percussion, the results of toluidine blue stain showed that most post-injury neurons were necrotic cells,the cells body were condensed,and nuclei were pycnosis and dark blue. The re- sults of immunocytochemical stain showed that there was some expression of TSP-1 in the neurons of control group, and the ex- pression of TSP-1 and the positive cells increased in injury group. The results of Western blot showed that the optical density value was 0.937 ~0. 194 in control group and 2. 318 ~0.495 in injury group,the expression of TSP-1 in the neurons of injury group was significantly stronger than that in control group（ P 〈 0.01 ）. Conclusion The increased expression of TSP-1 may play an important role in the development of neurons cultured in vitro by fluid percussion injury.

关 键 词：凝血酶敏感蛋白-1 神经细胞 液压冲击损伤 大鼠 

分 类 号：R338.2[医药卫生—人体生理学]