nPKCs在人肾小管上皮细胞的表达谱及尿蛋白对其表达的影响  

Novel Protein Kinase C Isoenzymes Expression Profiles and Urinary Proteins Influence on Its Expression in Human Renal Tubular Epithelial Cells

作　　者：杨陈[1] 尤永珂[1] 安宁[1] 唐荣[1] 陶静莉[1] 刘华锋[1] 

机构地区：[1]广东医学院附属医院肾脏疾病研究所,广东湛江524001

出　　处：《医学综述》2013年第4期719-722,共4页Medical Recapitulate

基　　金：广东省自然科学基金(815240231000014)

摘　　要：目的检测新型蛋白激酶C(nPKCs)同工酶在人肾小管上皮细胞(TECs)的表达谱及尿蛋白对其表达的影响。方法应用硫酸铵沉淀法提取局灶节段性肾小球硬化症患者尿液中的总蛋白成分;用纯化的尿蛋白在体外按不同浓度刺激人肾近曲小管上皮细胞(HK-2细胞),于24 h和48 h采用免疫细胞化学法检测HK-2细胞中nPKCs的表达变化。结果所提取尿蛋白的主要成分为白蛋白、转铁蛋白和IgG等,分别占72.1%、17.9%和9.5%,其他占0.5%;PKC-θ、PKC-δ和PKC-μ在尿蛋白刺激下表达量上调,而PKC-η与PKC-ε在刺激下表达量变化不明显。结论部分nPKCs可能参与尿蛋白致TECs的损伤过程。Objective To investigate expression profile of novel protein kinase C （PKC） isoenzymes the effects of urinary proteins on the expression in human renal tubular epithelial ceils（TECs）. Methods Uri- nary proteins were purified from patients with focal segmental glomerulosclerosis by ammonium sulfate precip- itation method. Human renal proximal tubule epithelial cells（ HK-2 cells）were stimulated with different doses of urinary proteins at 24 h and 48 h. The expression of novel PKC isoenzymes were detected by immunocyto- chemistry（ICC）. Results The main components of the extracted urinary protein were albumin, transferrin and IgG,respectively, accounted for 72.1%, 17.9% and 9.5% , others 0.5% ;the expression of PKC-0, PKC-8 and PKC-tx were up-regulated under stimulation by urinary proteins,while no effect on the expression of PKC-＇q and PKC-e was observed. Conclusion Some novel PKC isoenzymes may be involved in the pro- gress of renal TECs damage induced by urinary proteins.

关 键 词：新型PKC同工酶 尿蛋白 肾小管上皮细胞 

分 类 号：R692.6[医药卫生—泌尿科学]