丝裂霉素C对瘢痕疙瘩成纤维细胞TGF-β和Smads表达的影响  

Influence of mitomycin C on expressions of TGF-β/Smad in keloid fibroblasts

作　　者：孙奎[1] 吴晓明[1] 张鸿霞[1] 许新华[1] 王鹤鹏[1] 杨景哲[1] 李树松[1] 

机构地区：[1]承德医学院附属医院烧伤整形外科,河北承德067000

出　　处：《重庆医学》2013年第8期853-855,共3页Chongqing medicine

摘　　要：目的探讨丝裂霉素C(MMC)对瘢痕疙瘩成纤维细胞(KFB)转化生长因子β(TGF-β)/Smads通路作用及机制。方法采用不同浓度的MMC作用于体外培养的KFB,通过采用RT-PCR方法,检测MMC对体外培养的KFB TGF-β1mRNA表达的影响,采用Western blot技术检测MMC作用下体外培养KFB中Smad2/3、Smad4、Smad7蛋白的表达。结果 RT-PCR方法显示:MMC干预组TGF-β1mRNA相对表达量明显低于正常对照组,从12.5μg/L MMC开始具有显著的统计学差异(P<0.05),随MMC浓度增大,MMC干预组TGF-β1mRNA相对表达量呈逐渐降低趋势。Western blot技术检测显示:12.5μg/LMMC开始对KFB中Smad7蛋白的表达具有明显增强效应(P<0.05),50μg/L MMC对Smad2/3蛋白的表达开始有减弱效应(P<0.05),而对Smad4蛋白的表达无明显变化。结论 MMC对TGF-β1mRNA的表达具有明显减弱效应;加入不同浓度的MMC能明显增加KFB中Smad7蛋白的表达,减弱KFB中Smad2/3蛋白的表达,而对Smad4蛋白的表达无变化。Objective To study the effect of mitomycin C（MMC） on transforming growth factor（TGF）-β/Smad signal passage in keloid fibroblasts（KFB） and its mechanism. Methods The different concentrations of MMC were acted on in vitro cultured KFB and their influence on the expression of KFB TGF-β1 mRNA was detected by the RT-PCR method. The Western blot technology was used to detect the expression of Smad2/3,Smad4 and Smad7 protein in the in vitro cultured KFB under MMC action. Results The RT-PCR results showed that TGF-β1 mRNA related expression quantity in the MMC intervention group was significantly low- er than that in the normal control group,showing statistical differences starting from 12.5 μg/L MMC（P〈0.05）. With the MMC concentration increase,the related expression quantity of TGF-β1 mRNA in the MMC intervention group showed the gradually de- creasing trend. The Western blot detection displayed that 12.5 μg/L MMC started to show the enhancing effect on Smad7 protein expression in KFB（P〈0.05）, 50 vg/L MMC started the weakening effect on the expression of Smad2/3 protein（P〈0.05）, but had no effect on the Smad4 protein expression. Conclusion MMC has obviously decreasing effect on expression of TGF-β1 mRNA. The different concentrations of MMC can obviously increase the expression of Smad7 protein, decrease the expression of Smad2/3 protein in KFB and has no effect on the expression on Smad4 protein.

关 键 词：丝裂霉素C 药理学 瘢痕疙瘩 成纤维细胞 药理作用 转化生长因子β SMAD2/3 SMAD4 SMAD7 

分 类 号：R622[医药卫生—整形外科]