阿奇霉素对哮喘大鼠瘦素表达及气道炎症的影响  

Effects of azithromycin on expression of leptin and airway inflammation in rats with asthma

作　　者：朱述阳[1] 嵇桂娟[1] 卢立国[2] 闫明华[1] 段存玲 张文辉[1] 卞宏[1] 

机构地区：[1]徐州医学院附属医院呼吸内科,江苏徐州221006 [2]江苏省沭阳县人民医院呼吸科,223600

出　　处：《重庆医学》2013年第8期909-912,共4页Chongqing medicine

摘　　要：目的探讨阿奇霉素对肥胖哮喘大鼠气道炎症肺组织内及气道平滑肌细胞(ASMC)内瘦素表达的变化及抗炎作用。方法将大鼠随机分为正常对照组、正常哮喘组、正常干预组和肥胖对照组、肥胖哮喘组、肥胖干预组,建立肥胖、哮喘模型以及体外培养大鼠ASMC,采用酶联免疫吸附试验(ELISA)法测定血清、支气管肺泡灌洗液(BALF)及细胞上清液中瘦素浓度,Westernblot法检测肺组织和ASMC内瘦素蛋白的表达。结果肥胖对照组、肥胖哮喘组和肥胖干预组BALF白细胞总数及中性粒细胞数,肺组织瘦素蛋白表达及血清、BALF和细胞上清液中瘦素浓度与体质量正常相应组比较差异有统计学意义(P<0.05)。结论肥胖哮喘大鼠气道炎症肺组织内瘦素表达增加,阿奇霉素能抑制气道炎症并部分降低哮喘大鼠瘦素的表达。Objective To investigate the role of azithromycin on the expression change ot leptm in airway intlammatory lung tis- sues and airway smooth muscle cells（ASMC） in obese rats with asthma and its anti inflammatory effects. Methods Rats were ran- domly divided into 6 groups（n = 8） ：3 groups with normal weight including the control group（group A）, asthmatic group（group B）, and azithromycin intervention group（group C） and 3 groups with obesity including the control group（group D）,asthmatic group （group E） and azithromycin intervention group（group F）. The obese and asthma models were constructed. The rat ASMC was cul tured in vitro. The concentrations of leptin in serum, BALF and the supernatant of cultured ASMC were determined by ELISA, and the expression of leptin in lung tissue and ASMC was measured by Western blot. Results The total number of WBC and the num- ber of neutrophils of bronchoalveolar lavage fluid（BALF）,the protein expression of leptin in lung tissues and the leptin concentra- tions in serum,BALF and supernatant in the group D, E and F all showed statistical difference compared with the corresponding groups with normal weight（P〈0.05）. Conclusion The expression of leptin in the airway inflammatory lung tissues in obese rats with asthma is increased. Azithromycin can inhibit airway inflammation and partially reduce the expression of leptin in asthmatic rats.

关 键 词：支气管哮喘 肥胖症 气道平滑肌细胞 瘦素 阿奇霉素 

分 类 号：R562.25[医药卫生—呼吸系统]