黄脉爵床微体繁殖技术研究  

Researches on the micropropagation of Sanchezia nobilis Hook.f. in vitro

作　　者：蒋泽平[1] 李玉巧[1] 仲磊[1] 刘根林[1] 吴钢 

机构地区：[1]江苏省林业科学研究院,江苏南京211153

出　　处：《广西农业生物科学》2000年第4期228-232,共5页Journal of Guangxi Agricultural and Biological Science

基　　金：江苏省林业科学研究院青年基金课题!( 990 2 )

摘　　要：对黄脉爵床 ( Sanchezia nobilis Hook.f.)微繁技术进行了研究并获成功。研究结果表明 ,外源植物生长调节剂及营养物质对试管芽苗增殖生长及其不定根诱导均有显著影响。试管芽苗在MS+BA 0 .5mg/L+ZT 0 .5mg/L+NAA 0 .0 3mg/L +IAA 0 .0 3mg/L培养基上 ,可获得最大的单位芽苗数。 MS (大量元素减半 ,肌醇 1 /4量 ) +IBA 1 .0 mg/L (或 NAA0 .2 mg/L) +S330 70 .2 mg/L的培养基有利于芽苗生根 ;经 7d黑暗培养 ,可提高生根速率。以细河沙为基质 ,移裁成活率达 90Experimentsperformedtomicropropagate Sanchezianobilis Hook.f.gainedsuccess. The results revealed that the plant growth regulators and nutrients apparently influenced the multipli cation of the tubeshoots and the induction of adventitious roots.Most shoot number of tubeshoots per unit was obtained on MS medium supplemented with BA 0 5mg/L+ZT0 5mg/L+ NAA 0 03 mg/L+IAA 0 03 mg/L.The cultures on 1/2 MS mediumcontaining 1/4 Inositolwith 1 0 mg/L IBA(or 0 2 mg/L NAA) and 0 2 mg/L S3307 facilitated root formation.Rooting rate highly rose after a 7day dark incubation.The transplanting survival rate reached over 90% in fine river sand.

关 键 词：黄脉爵床 植物生长调节剂 微繁技术 观赏植物 

分 类 号：S682[农业科学—观赏园艺]