24种桉树无性系指纹图谱构建  

Establishment of Fingerprinting for 24 Clones in Eucalyptus

作　　者：张照远[1] 项东云[1] 邓紫宇[1] 唐庆兰[1] 陈健波[1] 兰俊 

机构地区：[1]广西林业科学研究院国家林业局中南速生材繁育实验室、广西优良用材林资源培育重点实验室,广西南宁530002 [2]广西东门林场,广西崇左532108

出　　处：《西北林学院学报》2013年第2期74-78,共5页Journal of Northwest Forestry University

基　　金：广西林科院基本科研业务费项目(林科200907号);国家林业公益性行业科研专项(201104003)

摘　　要：为有效鉴别和判定造林过程中无性系同一性,以24种常用的桉树无性系作为研究材料,利用RAPD分子标记技术构建相应指纹图谱。结果表明:利用引物S1001、S1009、S1065、S1076、S1156均能较好地鉴别试验材料,可作为这些常用无性系鉴定的理想分子标记引物。聚类分析显示U158与其他桉树无性系亲缘关系较远,其次是DH145和DH178,而DH33-27与DH32-22亲缘关系最近。To effectively identify and judge the consistency during clone afforestation,24 Eucalyptus clones were selected to establish fingerprinting by RAPD markers.The results showed that all the 24 studied materials could be distinguished by primer S1001,S1009,S1065,S1076,and S1156,respectively,which were selected as the ideal molecular marker primers to differentiate those clones in the future study.Cluster analysis showed that U158 presented further relationship with others,and the next was DH145 and DH178 that also had further relationship with each other.On the other hand,DH33-27 exhibited closer relationship with DH32-22.

关 键 词：桉树 无性系 指纹图谱 RAPD 

分 类 号：S792.39[农业科学—林木遗传育种]