MiR-143在ob/ob小鼠肝脏、骨骼肌中的表达及意义  

Expression of MiR-143 in liver and skeletal muscle of ob/ob mice and its significance

作　　者：张向君[1] 黄婷婷[1] 付海龙[1] 徐广峰 赵亚萍 王加林 

机构地区：[1]蚌埠医学院研究生部,安徽蚌埠233000 [2]解放军82医院

出　　处：《西南国防医药》2013年第3期244-246,共3页Medical Journal of National Defending Forces in Southwest China

摘　　要：目的观察miR-143在遗传性ob/ob肥胖小鼠与正常对照组C57/BL6J小鼠肝脏、骨骼肌中的差异表达,初步探讨miR-143在肥胖及相关脂代谢紊乱发生中的作用。方法选择16周龄的ob/ob小鼠与C57/BL6J小鼠各12只,采用实时荧光定量聚合酶链反应技术检测两组小鼠肝脏、骨骼肌中miR-143的表达水平。结果 miR-143在ob/ob小鼠肝脏中的表达水平明显高于C57/BL6J小鼠(P<0.05),但在骨骼肌中的表达水平两组无显著性差异(P>0.05)。结论 miR-143可能参与了ob/ob小鼠肝脏脂质代谢紊乱的发生。Objective To observe the different expression of miR - 143 in liver tissue and skeletal muscle of inherited ob/ob fatty mice and normal control mice （C57/BL6J）, and preliminarily explore the role of miR - 143 in obesity and disorder of related lipid metabolism. Methods Twelve ob/ob mice and 12 C57/BL6J mice at 16 - week - old were selected. Real - time fluorescent quantitative polymerase chain reaction technique was used to detect the expression level of miR - 143 in the liver and skeletal muscle of those mice. Results The expression level of miR - 143 in the liver of ob/ob mice was significantly higher than that in C57/BL6J mice （ P 〈 0.05 ） , but there was no significant difference in the expression in skeletal muscle between the two groups （ P 〉 0.05 ）. Conclusion MiR - 143 may participate the occurrence of liver lipid metabolism disorder in ob/ob mice.

关 键 词：MIR-143 ob/ob鼠 肥胖 脂质代谢紊乱 

分 类 号：Q522.2[生物学—生物化学] R322.74[医药卫生—人体解剖和组织胚胎学]