孔雀石绿对小鼠肝脏生理功能的影响  

Effect of malachite green on liver functions of mice

作　　者：张秀娟[1,2] 穆晶晶[1,2] 邢志华[3] 李其成[3] 贾绍华[3] 

机构地区：[1]哈尔滨商业大学生命科学与环境科学研究中心生物安全评价研究所,哈尔滨150076 [2]国家教育部抗肿瘤天然药物教育部工程研究中心,哈尔滨150076 [3]哈尔滨商业大学药学院,哈尔滨150076

出　　处：《哈尔滨商业大学学报（自然科学版）》2013年第1期4-7,共4页Journal of Harbin University of Commerce：Natural Sciences Edition

基　　金：黑龙江省教育厅项目(12521133);黑龙江省自然科学基金项目(D201043)

摘　　要：对孔雀石绿在哺乳动物体内蓄积后对其肝脏的影响进行研究.将昆明种小鼠随机分为四组:正常组、孔雀石绿5、10、20 mg/(kg.d)组,灌胃,正常组给予同体积生理盐水.30 d后,测定小鼠血清谷丙转氨酶(ALT)、谷草转氨酶(AST)及碱性磷酸酶(AKP)的变化、流式细胞仪检测小鼠肝细胞线粒体膜电位的变化、激光共聚焦显微镜检测肝细胞钙离子质量浓度的变化.与正常组相比孔雀石绿组小鼠血清中ALT、AST、AKP活性有所升高、肝细胞线粒体膜电位明显降低、肝细胞钙离子(Ca2+)质量浓度升高,且随着孔雀石绿量的加大,损伤程度增加.结果显示孔雀石绿在小鼠体内累积后可以通过影响肝细胞内酶活性、肝细胞线粒体膜电位及肝细胞内钙离子质量浓度的改变而导致肝脏损伤.This paper investigated the effect of malachite green on animal liver. Kunming mice were divided into four groups randomly ： normal group;malachite green, 5, 10, 20 mg/（ kg · d ） , ig , the normal group was given the same volume of saline. After 30 days, the change of alanine aminotransferase （ALT） , aspartate aminotransferase （AST） and alka- line phosphatase （AKP） in the serum were explored. The changes of hepatocytes mitochon- drial membrane potential were examined by flow cytometry . The changes in the calcium con- tent were measured by laser scanning confocal microscope. The results showed that the activ- ities of ALT , AST, and AKP in the serum were increased in the malachite green group which compared with the normal group, hepatocytes mitochondrial membrane potential was decreased significantly, the content of calcium in liver increased and with increasing of the a- mount of malachite green the degree of injury was increased. The malachite green to accumu- late in mice can affect the change of hepatocytes enzyme activities, mitochondrial membrane potential and hepatocytes calcium content which lead to liver damage ultimately.

关 键 词：孔雀石绿 转氨酶 肝细胞 线粒体膜电位 [CA2+]I 

分 类 号：R285[医药卫生—中药学]