雌激素对人胆管上皮细胞增殖作用的体外研究  

Effect of 17β-estradiol on proliferation of human normal hiliary epithelial cells in vitro

作　　者：所广车[1] 朱哲[1] 赵中辛[1] 

机构地区：[1]同济大学附属东方医院胃肠外科,上海200120

出　　处：《腹部外科》2013年第1期59-61,共3页Journal of Abdominal Surgery

基　　金：上海浦东新区科技发展基金项目（PKJ2009-Y21）

摘　　要：目的研究雌激素对体外培养的人胆管上皮细胞增殖的影响，并探讨其可能的作用机制。方法体外培养人胆管上皮细胞株，加入雌激素17β-雌二醇作用于人胆管上皮细胞，用CCK8方法检测17p雌二醇对人胆管上皮细胞生长的影响；Westernblot法检测胆管上皮细胞的雌激素受体（ER-a、ER-β）表达水平；并通过酶联免疫吸附试验（ELISA）法检测上述两种培养条件下胆管上皮细胞培养上清中表皮细胞生长因子（EGF）的分泌水平。将雌激素组与无雌激素组（对照组）结果进行比较。结果雌激素可以促进人胆管上皮细胞增殖，雌激素组的人胆管上皮细胞ER—a受体表达水平明显高于对照组（P〈0．05），两组上清中的EGF水平差异无统计学意义（P〉0．05）。结论雌激素17β-雌二醇可以诱导人胆管上皮细胞增殖，为其作用于胆管上皮细胞的雌激素受体而发生生物学效应提供了进一步证据，此外，我们的研究提示雌激素17β-雌二醇可以诱导人胆管上皮细胞增殖与表皮细胞生长因子通路没有相关性。[Abstract] Objective To explore the effect of estrogen on proliferation of human biliary epithelial ceils （HBECs） cultured in vitro and the possible action mechanism. Methods In vitro culture HBECs were treated with 17β-estradiol. The effects of 17β-estradiol on proliferation of HBECs were assayed by using CCK-8. The expression level of the estrogen receptors （ER-a and ER-β） in HBECs was detected by using Western blotting. The epidermal growth factor （EGF） secretion levels in the culture supernatant of HBECs were examined by using ELISA in HBECs. Results 17β-estradiol could promote proliferation of HBECs. In estradiol group the ER-alpha expression level was significantly higher than that in the control group （P〈0. 05）, but the EGF levels in the culture supernatant of the both groups had no significant difference （P〉0. 05）. Conclusion 17β-estradiol induced HBECs proliferation, mainly through the estrogen receptors, rather than through the EGF pathway.

关 键 词：雌二醇 人类 胆管 上皮细胞 受体 雌激素 表皮生长因子 

分 类 号：R657.4[医药卫生—外科学]