3种不同方法对入境旧书中携带蜡样芽孢杆菌的检测分析  

Analysis on three kinds of different methods for indentifing Bacillus cereus in imported secondhand book

作　　者：胡群[1] 马研[1] 童淑梅[1] 马思杰[1] 雷磊[1] 邹春颖[1] 

机构地区：[1]大榭出入境检验检疫局公共卫生实验室,浙江宁波315812

出　　处：《中国国境卫生检疫杂志》2013年第1期24-26,共3页Chinese Journal of Frontier Health and Quarantine

摘　　要：目的对一株从美国入境旧书中分离的菌株进行鉴定。方法从旧书表面采集样本分离获得一株菌株,分别采用VITEK生化鉴定、16s rDNA序列分析法及特异性引物PCR 3种方法进行鉴定。结果VITEK生化鉴定判定可能属于蜡样芽孢杆菌、苏云金芽孢杆菌或蕈状芽孢杆菌的一种。16s rDNA通用引物PCR检测结果显示该菌株与芽孢杆属同源性最高达99%。特异性引物PCR检测结果判定为蜡样芽孢杆菌。结论初筛和特异性检测方法相结合,可以有效的对入境货物携带的致病微生物进行鉴定。Objective To indentify a bacterium strain from American imported secondhand book. Methods The samples from secondhand book surface were collected, and a bacterium strain from the samples was isolated, then the strain was identified by three methods of VITEK biochemical identification, 16s rDNA sequence analysis and special primer PCR. Results VITEK biochemical identification result showed the strain belong to one of Bacillus cereus, Bacillus thuringiensis, Bacillus mycoides. 16s rDNA sequence analysis result showed that strain shared 99% 16S ribosomal DNA sequence homology with Bacillus sp. Special primer PCR showed the strain was identified as Bacillus cereus. Conclusion The combination of screening and special detection methods can be effective for identifing disease-causing microorganisms carried by imported goods.

关 键 词：蜡样芽孢杆菌 检测方法 入境货物 生化鉴定 聚合酶链式反应 

分 类 号：R115[医药卫生—公共卫生与预防医学]