核桃体细胞胚发生与转基因研究进展  

AN OVERVIEW OF PROGRESS ON SOMATIC EMBRYOGENESIS AND TRANSFORMATION IN WALNUT

作　　者：汤浩茹[1] 王永清[1] 任正隆[2] 

机构地区：[1]四川农业大学林学园艺学院,雅安625014 [2]四川农业大学植物遗传育种省级重点实验室,雅安625014

出　　处：《林业科学》2000年第3期102-110,共9页Scientia Silvae Sinicae

摘　　要：总结了核桃体细胞胚发生的研究进展 ,列表统计已报道的核桃 5个种和 3个杂种体细胞胚发生的外植体与诱导条件 ,重点论述了影响核桃体细胞胚发生与次生胚发生的因素 ,介绍了核桃体细胞胚萌发与转化的方法。还总结核桃转基因研究的进展 。Significant progress on the somatic embryogenesis and transformation in walnut (Juglans) has been made in the past decade.Somatic embryogenesis has been achieved in 5 species of walnut.Cotyledons and zygotic embryos from immature fruit were routinely used as the explants for inducing somatic embryogenesis of walnut.Repetitive somatic embryogenesis has been used for the clonal production of in vitro plants and for gene transformation and regeneration of transgenic plants.Using Agrobacterium \|mediated transformation of somatic embryos as a method,genes of interest have been transformed into walnut.The transgenic plants successfully expressed the transformed genes.An overview of the progress on those areas in walnut was presented in this article.It focuses on the points of induction of somatic embryogenesis and repetitive somatic embryogenesis as well as somatic embryos germination and their conversion.A model of using Agrobacterium \|mediated transformation of somatic embryos for the production of transgenic plants in walnut was also presented.

关 键 词：核桃 体细胞胚发生 转基因 离体培养 

分 类 号：S664.103.6[农业科学—果树学]