几种农作物对绿黄隆敏感性的生物测定及在残留检测中的应用  

BIOASSAY METHOD OF SENSITIVITY OF SOME CROPS TO CHLORSULFURON AND ITS APPLICATION IN RESIDUE ANALYSIS

作　　者：袁树忠[1] 刘曙照[1] 李国生[1] 张建军[1] 戴忠军 孔勇 

机构地区：[1]扬州大学农学院植保系,江苏扬州225009

出　　处：《江苏农业研究》2000年第2期62-66,共5页

基　　金：国家自然科学基金资助项目 !( 3 9670 497)

摘　　要：采用土壤添加培养的方法测定了绿黄隆对玉米、水稻、大豆等作物的生物活性。结果表明 :绿黄隆在土壤中对玉米的致害阈限 ( IC1 0 )为 0 .1 7μg/ kg,对水稻的致害阈限则为 0 .4 3 4 9～ 0 .94 1 0μg/ kg,同时 ,绿黄隆的代谢产物之一的邻氯苯磺酰胺对玉米的生物活性要比绿黄隆的低得多 ,它对以玉米根系鲜重为指示指标的生物测定方法不会产生干扰 ,确定了以玉米根系鲜重为指示指标的生物测定方法 ,并以此得到了标准方程 ( P=4 .6653 +1 .0 2 3 5lg C,r=0 .9859* * ) ,用该方法检测了田间连续 2年使用绿黄隆后土壤中的残留量。检测结果表明 :第 1年使用 ,6个月后土壤中绿黄隆的残留量约为施药当日的 1 0 %左右 ;连续 2年使用 。The bioactivity of chlorsulfuron to maize, rice, soybean and rape was tested in 1996~1998. The results showed that the sensitivity of maize to chlorsulfuron was higher than that of the others to it. Growth of maize root system was extremely susceptible to chlorsulfuron. Its fresh weight was reduced to 10% at chlorsulfuron 0.17 μg/kg (dry soil). 2 chlorophenysulfonylamide (CPSA), the main metabolite of chlorsulfuron, didn′t interfere with the bioassay because CPSA didn′t inhibit the growth of maize root or bud until its concentration reached to 5~10 μg/mL. The inhibition of chlorsulfuron to rice root was more serious than that of chlorsulfuron to rice bud or sheath leaf. For this reason, the standard formulae ( P=4.665 3+1.243 5 lg C, r =0.985 9 ** , C =0.1~10.0 μg/kg) was established by the bioassay method with the index of fresh weight of maize root system, and the chlorsulfuron residues in cropland was assayed. The residues, after 6 months, were about 10% of chlorsulfuron concentration application in soil. They were slightly increased when chlorsulfuron was applied again for the second year.

关 键 词：作物 绿黄隆 生物测定 残留检测 

分 类 号：S451.2[农业科学—植物保护] S482.4