大鼠马钱子碱中毒肝细胞Bcl-2和Caspase-3蛋白表达的研究  

Expressions of Bcl-2 and caspase-3 in rate liver cells after brucine poisoning

作　　者：雷怀成[1] 朱方成[1] 魏来[1] 

机构地区：[1]湖北医药学院法医学教研室,十堰442000

出　　处：《陕西医学杂志》2013年第3期278-280,288,共4页Shaanxi Medical Journal

摘　　要：目的:研究马钱子碱中毒后不同时间段内肝细胞Bcl-2和Caspase-3表达的病理变化。方法:用20只Wistar大鼠为正常对照组,40只Wistar大鼠复制马钱子中毒模型,用免疫组织化学技术对中毒后大鼠肝细胞Bcl-2和Caspase-3的蛋白表达进行检测,并用图像分析对检测结果进行分析。结果:大鼠在马钱子中毒低、中剂量后的1～7d不同时间段内,肝细胞Bcl-2和Caspase-3蛋白阳性表达数均明显高于正常对照组,中毒组与对照组相比,差异有显著性。结论:Bcl-2和Caspase-3参与了马钱子碱中毒的病理生理过程。Objective：To study the expressions of Bcl-2 and Caspase-3 protein in rate liver cells after brucine poisoning at different stages. Methods：Sixty Wistar rates were divided into normal group, brucine poisoned group, and the expression level of Bcl-2 and Caspase-3 protein in liver cells were investigated using immunohistochemistry. The regular HE staining and immunohistochemical staining results were analyzed by computer image analysis competitive system. Results： Expression of Bcl-2 and Caspase-3 protein in liver cells in poisoning groups were much more than those in control ones at different stages. Conclusion：Bcl-2 and Caspase^3 proteins may participate in the pathophysiological procedure of brucin-poi soned rats.

关 键 词：碱中毒 病理生理学 马钱子 基因BCL-2 免疫学 半胱氨酸天冬氨酸蛋白酶3 免疫学肝细胞基因表达 1000—7377(2013)03—0278—04 

分 类 号：R392.5[医药卫生—免疫学]