糖尿病患者尿层粘连蛋白的测定  

Determination of urinary laminin in diabetes mellitus

作　　者：龚进 刘丁[2] 陈伟[2] 

机构地区：[1]重庆市第六人民医院,邮政编码400060 [2]第三军医大学第三附属医院

出　　处：《中国糖尿病杂志》2000年第5期292-294,共3页Chinese Journal of Diabetes

摘　　要：目的　探讨尿层粘连蛋白 (L N)与糖尿病肾病的关系。方法　将 PEG- 40 0 0和 γ-球蛋白浓缩的尿标本 ,用放射免疫法检测了 83例糖尿病患者的尿 L N含量 ,同时测定了尿微量白蛋白 (M- Alb)、肌酐清除率 (Ccr)、血糖 (Glu)和糖化血红蛋白 (Hb A1c)。结果　选用 15 0 g/ L的 PEG- 40 0 0和 0 .5 %的γ-球蛋白 ,二者具有良好的浓缩功能 ,糖尿病患者尿L N均较正常对照组高。结论　尿 L N可作为诊断早期糖尿病肾病的敏感指标。Objective To study the relationship between urinary laminin(LN) and diabetic nephropathy.Methods Using the method for concentrating urinary laminin based on precipitation with PEG-4 000 and γ-globulin.The concentration of LN in the urine of 83 diabetics was measured by RIA.The concentration of micro-albumin and Ccr in the urine and the levels of plasma glucose and HbA1c were also measured.Results The choice of 150g/L of PEG-4 000 and 0.5 g/L of γ-globulin was the best method for concentrating urinary LN.The urinary LN in all groups was significantly higher than that in control group.Conclusion Urinary LN is an indicator in the diagnosis of the early diabetic nephropathy.

关 键 词：层粘连蛋白 糖尿病 诊断 

分 类 号：R587.1[医药卫生—内分泌]