脱氢异雄酮对高糖下视网膜毛细血管周细胞增殖的影响  

The effect of dehydroepiandrostrone to retinal capillary pericytes under high glucose

作　　者：程丽霞[1] 董砚虎[1] 王家富 

机构地区：[1]山东潍坊医学院内分泌研究室,261042

出　　处：《中国糖尿病杂志》2000年第5期295-297,共3页Chinese Journal of Diabetes

摘　　要：目的　研究脱氢异雄酮 (DHEA)对高糖下视网膜毛细血管周细胞增殖的影响。方法　以新鲜牛眼球为材料 ,分离并培养周细胞 ;采用 3H- Td R掺入法及液体闪烁技术 ,研究不同浓度的 DHEA(10、5 0、10 0、5 0 0、10 0 0 nmol/L)对周细胞增殖的影响。结果　高浓度葡萄糖 (2 0 mmol/L)培养液中加入 DHEA(5 0、10 0、5 0 0、10 0 0 nmol/L)培养周细胞 48小时 ,周细胞的增殖与对照组比较均有显著性差异 ,其中 DHEA为 5 0 0 nmol/L时对周细胞增殖的促进作用最大 (增殖率为42 .6 7% )。结论　 DHEA可促进高糖下周细胞的增殖 ,这为筛选临床防治糖尿病视网膜病变的有效药物提供了可靠的依据。Objective To observe the effect of dehydroepiandrostrone (DHEA) on retinal capillary pericytes' proliferation under high glucose and research the prevention and treatment of diabetic retinopathy (DR).Methods Retinas were dissected from eyes of fresh killed cattle and retinal capillary pericytes were separated and cultured.The effect of DHEA (10、50、100、500、1 000nmol/L) on pericytes' proliferetion was evaluated by 3 H-thymidine incorporation and li- quid scintillation techniques.Results There was significant difference between DHEA (50、100、500、1 000nmol/L) and control culturing for 48h.The increasing rate was 42.67% in DHEA of 500nmol/L.Conclusion DHEA could promote pericytes' proliferation under high glucose.This provided reliable evidence for selecting effective drugs for prevention and treatment of DR clinically.

关 键 词：毛细血管周细胞 葡萄糖 糖尿病视网膜病变 

分 类 号：R587.2[医药卫生—内分泌] R774.1[医药卫生—内科学]