ATP对噪声暴露豚鼠耳蜗一氧化氮合酶活性的影响  

The Effects of ATP on Nitric Oxide Synthase in Noise Exposure Cochlea of Guinea Pigs

作　　者：沈静[1] 汤浩[1] 章晓芳 张合 张铁民 宋玉梅 

机构地区：[1]沈阳中国医科大学听力研究室,110001

出　　处：《听力学及言语疾病杂志》2000年第4期207-210,共4页Journal of Audiology and Speech Pathology

摘　　要：目的　探讨ATP对噪声暴露听力损伤的保护作用及对耳蜗一氧化氮合酶活性的影响。方法　制备豚鼠稳态噪声暴露听力损伤模型 ;应用NADPH -d酶组织化学及免疫组化技术ABC法 ,结合听性脑干反应 (ABR)阈值检测 ,研究ATP对噪声暴露致聋豚鼠耳蜗一氧化氮合酶 (NOS)及诱生型一氧化氮合酶 (iNOS)活性的影响。结果　ATP对噪声暴露豚鼠的ABR阈移有明显的抑制作用 ;ATP对噪声暴露豚鼠耳蜗NOS、iNOS的活性升高有明显抑制作用。结论　ATP对噪声暴露所致听力损伤具有保护作用 ,这种作用可能与耳蜗NOS。Objective To explore the protective effects of ATP on noise exposure hearing loss and the effects on nitric oxide synthase of cochlea.Methods Preparing the animal model of noise-induced hearing loss,using the NADPH-d enzymatic histochemistry method and ABC immunohistochemistry method for iNOS analysis,and ABR threshold.Results ATP decreased the enhancing of ABR threshold by exposed to noise.ATP decreased the increased activity increasing both of NOS and iNOS after exposed to noise.Conclusion ATP could resist the injure of cochlea by noise exposure,and this effect may be involved in the decreasing of NOS(iNOS)activity.

关 键 词：三磷酸腺苷 噪声 一氧化氮合酶 诱生型 听力损伤 

分 类 号：R764.432[医药卫生—耳鼻咽喉科]