银杏提取物对家兔缺血-再灌注心功能和心肌P-S及NOS表达的影响  

The Effects of GBE on Cardiac Function and Expression of P-S and NOS during Ischemia/Reperfusion of Myocardium in Rabbits and Its Possible Mechanisms

作　　者：张蓓[1] 秦际德[2,3,4] 刘兴德[2] 

机构地区：[1]贵阳医学院附院超声中心,贵州贵阳550004 [2]贵阳医学院附院心血管内科,贵州贵阳550004 [3]贵阳医学院 [4]河南商丘市第一人民医院心内科一病区

出　　处：《贵阳医学院学报》2013年第1期16-18,23,共4页Journal of Guiyang Medical College

基　　金：贵州省高层次人才科研条件特助经费资助项目;TZJF(2006)12号

摘　　要：目的:观察银杏提取物(GBE)对心肌缺血-再灌注(I/R)家兔心功能及心肌血管内皮细胞中P-选择素(P-S)、一氧化氮合酶(NOS)表达的影响。方法:家兔(24只)随机分为3组,假手术组(Sham组)、心肌缺血再灌注组(I/R组)及GBE干预组(GBE组),记录各组家兔I/R 180 min时的心脏左室收缩压(LVSP)、左室舒张末压(LVEDP)和左室内压最大收缩-舒张变化速率(±dp/dtmax),用RT-PCR技术检测P-S、NOS。结果:与Sham组比较,I/R组LVSP和±dp/dtmax显著降低,LVEDP显著增高,有统计学意义(P<0.05);与I/R组比较,GBE组LVSP和±dp/dtmax显著增高,LVEDP显著降低,有统计学意义(P<0.05)。与I/R组比较,GBE心肌血管内皮细胞P-S表达显著减少,而NOS表达显著增多,有统计学意义(P<0.05)。结论:GBE能够改善心肌I/R家兔的心功能,这可能与下调血管内皮细胞P-S的表达、上调NOS的表达有关。Objective： To investigate the effects of Ginkgo biloba extract （GBE） on cardiac function and expression of P-selection （P-S） and NOS in vascular endothelium cells of myocardium during ischemia/reperfusion （I/R） in rabbits. Methods： Twenty-four rabbits were randomly divided into 3 groups （8 in each） ： Sham-operated group （Sham）, ischemia/reperfusion group （I/R）, and GBE intervention group （GBE）. Left ventricular systolic pressure （LVSP）, left ventricular end-diastolic volume （LVEDP）, and maximal changing rate of left ventricular inner （ ± dp/dtmax） of rabbits in 180min after I/R were recorded. Immunohistochemical method was used to detect expression of P-S ion and NOS in myocardium. Results： Of group I/R, LVSP and ± dp/dtmax were significandy lower, and LVEDP was significantly higher than those of group Sham （P 〈 0. 05 ） ; In group GBE, LVSP and ± dp/dtmax were remarkably higher, but LVEDP was remarkably lower than those in group I/R （P 〈 0. 05） ; Compared with that of group I/R, LVSP and ±dp/dtmax of group GBE increased remarkably, while LVEDP decreased remarkably （P 〈 0. 05 ）. Compared with that of group I/R, P-S expression of group GBE decreased remarkably , while eNOS increased remarkably （ P 〈 0. 05 ）. Conclusion： GBE could improve cardiac function during myocardium I/R in rabbits. The mechanisms might be down regulation of P-S expression in vascular endothelium ceils, and up regulation of eNOS expression.

关 键 词：银杏提取物 心功能 再灌注损伤 P-选择素 一氧化氮合酶 

分 类 号：R543.31[医药卫生—心血管疾病]