氢气生理盐水对大鼠肝缺血再灌注后氧化应激损伤的保护作用  被引量:3

Protection effects of hydrogen-rich saline for hepatic ischemia-reperfusion injury by reducing oxidative stress in rats

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作  者:骆助林[1] 汤礼军[1] 汪涛[1] 罗浩[1] 黄竹[1] 王华[1] 田伏洲[1] 

机构地区:[1]成都军区总医院普外科,四川610083

出  处:《创伤外科杂志》2013年第2期157-159,共3页Journal of Traumatic Surgery

基  金:全军医学科学技术研究"十一五"计划项目(08Z012)

摘  要:目的研究氢气生理盐水(Hydrogen-rich saline,HS)抑制氧化应激,减轻大鼠肝缺血再灌注损伤(IRI)的作用。方法将32只雄性Wistar大鼠随机分为假手术组(sham)、肝缺血再灌注组(IR)、生理盐水处理组(IR+NS)和氢气生理盐水处理组(IR+HS),制作70%大鼠肝脏缺血再灌注模型,术后12h处死大鼠,检测各组大鼠血清丙氨酸转氨酶(AIR)和天冬氨酸转氨酶(AST)水平;取肝组织观察其病理学变化,并观察肝组织内超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽(GSH)变化。结果与IR+NS组相比较,术后IR+HS组肝组织病理损伤情况明显改善,血清AIR及AST值较低(P<0.05),肝组织MDA水平明显下降(P<0.05),SOD活性和GSH含量明显上升(P<0.05)。结论 HS能减轻大鼠肝IRI,其机制可能与抑制肝缺血再灌注后氧化应激反应有关。Objective To investigate the protective effects of hydrogen-rich saline for hepatic ischemia-reperfusion injury by inhibiting the oxidative stress in rats. Methods Thirty two male Wistar rats were randomly allocated into 4 groups : sham operation group, ischemia - reperfusion ( IR ) group, IR + normal saline ( IR + NS ) group and IR + hydrogen-rich saline ( IR + HS ) group. A rat model of hepatic ischemia-reperfusion injury was established in this experiment. After operation for 12 hours, the rats were sacrificed and the serum and liver samples were collected. The AIR and AST levels in serum were determined, liver histological damage was evaluated, and the superoxide dismutase( SOD ), Malondialdehyde (MDA) and glutathione ( GSH ) were also determined. Results The pancreas tissue in IR + HS group was more slightly damaged than those in IR + NS group after operation. The AIR, AST level in IR + HS group were significantly lower than those of the IR + NS group ( P 〈 0.05 ). The levels of plasma MDA were lower in IR + HS group than those in IR + NS group ( P 〈 0.05 ). The levels of plasma SOD and GSH were higher in IR + HS group than those in IR + NS group ( P 〈 0.05 }. Conclusion Hydrogen-rich saline may reduce the damage of hepatic ischemia-reperfusion injury by inhibiting oxidant stress in rats.

关 键 词:氢气 氧化应激 肝缺血 再灌注 大鼠 模型 

分 类 号:R453[医药卫生—治疗学]

 

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