丝素蛋白-壳聚糖三维多孔支架材料生物相容性及促成骨性能研究  

Biocompatibility and osteogenesis of silk fibroinitosan scaffold

作　　者：柳磊[1] 曾曙光[2] 石勇[2] 容明灯[2] 高文峰[2] 郭泽鸿[2] 许竞[2] 

机构地区：[1]中山市人民医院口腔科,广东中山528403 [2]南方医科大学附属口腔医院.广东省口腔医院

出　　处：《广东牙病防治》2013年第2期75-80,共6页Journal of Dental Prevention and Treatment

基　　金：广东省中医药局科研基金资助项目(20111145);广东省科技计划项目(2011B031300022)

摘　　要：目的研究丝素蛋白-壳聚糖支架材料的生物相容性及成骨性能,为其在骨组织工程方面的应用提供理论基础。方法采用冷冻干燥合并化学交联的方法制备出2组支架材料,实验组采用丝素蛋白-壳聚糖支架(质量比为2∶3),对照组采用壳聚糖支架。将MG-63成骨细胞接种于这2组支架上,通过Hoechst荧光染色观察其在支架材料上的生长,计算细胞粘附率和增殖率,最后通过矿化结节的形成以及MG-63成骨细胞分泌碱性磷酸酶能力来研究支架的成骨性能。以载玻片培养MG-63细胞为空白对照组。结果细胞培养1、3 d时,对照组细胞粘附率分别为(26.63±0.57)%、(49.88±0.78)%,实验组细胞粘附率分别为(31.88±0.94)%、(62.13±0.36)%,实验组的细胞粘附率高于对照组,差异有统计学意义(P<0.05)。细胞培养3 d时,对照组和实验组细胞增殖率检测的光密度值分别为0.491±0.010、0.596±0.008,实验组的细胞增殖率高于对照组,差异有统计学意义(P<0.05)。实验组碱性磷酸酶活性和矿化结节的生长状况也均优于对照组。结论丝素蛋白-壳聚糖支架(质量比为2∶3)具有良好的生物相容性和优良的促成骨性能,对于骨组织工程研究有良好的应用前景。Objective Co-cuhL,re of silk fibroin（SF）/chitosan（CS） scaffold and MG-63 osteoblast cells in vitro andinvestigate biocomlpatibility and osteogenesis to demonstrate the preliminary feasibility of the bone tissue engineering. Methods Prepared scaffolds with freezing -drying and chemical crosslinking methods, and divided the subjects into40% SF-60% CS group （ experimental group） and 100% CS group （ control group）. Observed the growth of MG-63 osteoblast cells in the scaffolds using Hoechst fluorescence staining and the biocompatibility of the materials by the way of calculating adhesion rate and proliferation rate. Lastly, explored the osteogenesis by the evaluatiion of formation mineralization nodules and promoting sereetion of alkaline phosphatase（ALP） by MG-63 ceils. Blank control group only used glassslides to culture. Results The cell adhesion rate, the cell proliferation rate, the promotion of MG-63 （;ell to secret alkaline phosphatase, the resuh of mineralization indices including the MG-63 cells union, ground substance secretion and the formation of calcified nodule, all indicated that the group of 40% SF-60% CS surpassed the 100% CS group. Conclusion The 40% SF-60% CS scaffold have good biocompatibility and can promote the biological function of osteoblast, showingwide application prospects as bone tissue engineering in the future.

关 键 词：丝素蛋白 壳聚糖 骨组织工程 生物相容性 成骨细胞 

分 类 号：R318.08[医药卫生—生物医学工程]