肝脏低温保存再灌注肝细胞凋亡及其与细胞内钙离子浓度的相关性  

Experimental study on hepatic cell apoptosis and its relationship with intracellular [Ca^(2+)] during cryopreservationreperfusion of isolated livers

作　　者：汤礼军[1] 田伏洲[1] 王雨[1] 李晓军[1] 

机构地区：[1]成都军区总医院全军普外中心实验室,四川成都610083

出　　处：《中国危重病急救医学》2000年第7期406-409,共4页Chinese Critical Care Medicine

摘　　要：目的 :研究肝移植过程中供肝低温保存再灌注期间肝实质细胞凋亡及其相关机制。方法 :应用细胞凋亡原位末端标记法并结合电镜观察 ,检测不同低温保存时间 (分别为 0、3、6和 9小时 )的 4组兔肝脏在低温保存再灌注过程中肝实质细胞凋亡发生情况。同时 ,对各组肝脏再灌注前、后肝实质细胞内游离钙离子浓度([Ca2 + ]i)进行测定。结果 :各组肝脏于低温保存末 ,组织内未见或仅偶见肝实质细胞凋亡现象 ;而低温保存后的再灌注期间 ,组织内可见明显的肝实质细胞凋亡现象 ,且低温保存时间越长的肝脏 ,再灌注后凋亡细胞数量越多。此外 ,4组肝脏肝实质细胞凋亡数量与肝实质细胞内 [Ca2 + ]i呈显著的正相关关系。结论 :肝实质细胞凋亡明显参与了肝脏低温保存再灌注损伤过程 ,而肝实质细胞 [Ca2 + ]超载可能是其凋亡的重要机制。Objective:To investigate the nature and possible mechanisms of hepatic cell apoptosis during cryopreservationreperfusion in the process of liver transplantation.Methods:Four groups of rabbit livers,which were cryopreserved for 0,3,6,9 hours respectively,were taken into observation.Apoptosis of hepatic parenchymal cells were analyzed using TdTmediated dUTPbiotin nick end labeling and electron microscope.Levels of intracellular [Ca 2+ ] of hepatic parenchymal cells were observed at the time points of both prereperfusion and postreperfusion.Results:Apoptotic hepatic parenchymal cells rarely appeared at the endpoint of cryopreservation in each group but they were obviously seen in reperfusion livers subsequent to cryopreservation.The longer the liver cryopreserved,the greater the number of apoptotic cells.In addition,there was significant positive correlation between the number of apoptotic hepatic parenchymal cells and the intracellular [Ca 2+ ].Conclusions:Apoptosis of hepatic parenchymal cells exists in the process of donor liver cryopreservationreperfusion and intracellular [Ca 2+ ] overloading might play an important role in inducing such apoptosis.

关 键 词：肝脏 低温保存 再灌注损伤 钙离子浓度 肝移植 

分 类 号：R657.3[医药卫生—外科学]