汉坦病毒A9株L片段部分核苷酸序列分析  

The partial nucleotide sequence of L segment of Hantavirus strain A9

作　　者：李德新[1] 梁米芳[1] 王晓芳[1] 李川[1] 张全福[1] 霍子威[1] 抗长寿 宋干[1] 

机构地区：[1]中国预防医学科学院病毒学研究所出血热研究室,北京100052

出　　处：《中华实验和临床病毒学杂志》2000年第2期109-112,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：国家 8 63计划基金资助!( 10 2 0 7 0 2 0 3 )

摘　　要：目的　测定我国分离的汉坦病毒A9株L片段的部分核苷酸序列。方法　用逆转录 聚合酶链反应 (RT PCR)技术扩增我国分离的汉坦病毒A9株部分L片段cDNA ,测定PCR产物的核苷酸序列。结果　PCR扩增产物为L片段 40 0 6nt 44 5 41nt(根据 76 118株序列 ) ,和已发表的汉坦病毒L片段序列比较 ,A9株和汉滩病毒 76 118株同源性最高 ,为 85 .2 % ,和其他不同型别汉坦病毒代表株HR80 39、CG182 0、Sotkoma、NHR11、TulaL片段的同源性分别为 76 .8%、72 .3%、71.0 % ,71.7%和 70 .8%。比较推测的氨基酸序列 ,和 76 118株同源性为 96 .7% ,和HR80 39、CG182 0、Sotkoma、NHR11、TulaL片段的同源性分别为 88.3%、78.6 %、74.7% 74.0 %和 76 .0 %。遗传进化分析显示A9株L片段的进化和同为亚洲黑线姬鼠分离的 76 118株较密切 ,和从欧洲、美洲其他动物分离病毒相差较远 ,提示汉坦病毒的进化和宿主动物密切相关。结论　汉坦病毒A9株和其他不同型别汉坦病毒L片段核苷酸序列的同源性在 85 %～ 70 .8%之间 ,和汉坦病毒原型株 76 118株的同源性最高。Objective To study the nucleotide sequence of L segment of Hantavirus strain A9.Methods The partial L segment cDNA of A9 strain was amplified by PCR, the PCR product was sequenced.Results The nucleotide sequence of partial L segment of A9 strain was provided, the sequence was A, T rich,similar to other Hantaviruses, and similar to both M, S segments.Comparied with nucleotide sequences from other Hantaviruses, it revealed a higher homology to HTN virus strain 76 118,83.5% at nucleotide level and 96.7% at deduced amino level.SNV, PUU and Tula viruses shared the lower homology.A9 and 76 118 were at same cluster in phylogenetic analysis, close to SEO virus but not to PUU, SNV, Tula viruses.Comparing of partial nucleotide sequence of strain A9 with 76 118, some conserved regions were found, but also some regions of A9 were more conserved to other viruses not 76 118.It suggested that when design the primers for L segments amplification, more than one type of Hantavirus sequence should be concerned.Like the homology from M and S segments of Hantaviruses, the viruses isolated from same host animal showed high homology.Conclusion Nucleotide sequence of L segment of Hantavirus strain A9 revealed homology between 85.2% 70.8% to different types of Hantavirus and shared a higher homology of 85.2% to strain 76 118.

关 键 词：汉坦病毒 基因 碱基序列 

分 类 号：R373[医药卫生—病原生物学]