苦参对感染柯萨奇B3病毒乳鼠搏动心肌细胞的保护作用  

Effect of sophora flavescens Ait on cultured beating myocardial cells of coxsackie B3 virus infected newborn rat

作　　者：陈曙霞[1] 彭旭[1] 刘晶星[1] 丁智勇 

机构地区：[1]上海第二医科大学附属仁济医院心内科,上海200001

出　　处：《中华实验和临床病毒学杂志》2000年第2期137-140,共4页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的　研究苦参中对抗柯萨奇B3病毒 (CVB3)的有效成份 ,即抗柯萨奇注射液 (SFA)抗CVB3病毒的作用。方法　将心肌细胞分为 4组 ,感染组 (n =8) :只感染病毒 ,不加SFA ;治疗组 (n =8) :感染病毒后加入SFA(10 0mg/L) ;药物组 (n =6 ) :不感染病毒 ,只加入SFA(10 0mg/L) ;对照组 (n =6 ) ;不感染病毒 ,不加SFA。结果　在感染病毒后第 2、3、5d ,感染组心肌细胞的病变程度较其余 3组重 ,乳酸脱氢酶 (LDH)与谷草转氨酶 (SGOT)的浓度明显增高 ,与其余 3组比较差异有显著性 (P <0 .0 5 ) ,心肌细胞中病毒滴度比治疗组高 2 5 .12～ 15 8.5 0倍。SFA在 30 0mg/L浓度下对正常心肌细胞的结构和功能无影响 ;在 6 .2 5～ 2 0 0mg/L范围内对感染病毒的心肌细胞具有保护作用。结论　SFA可以抑制CVB3在心肌细胞中的复制。Objective Coxsackie B viruses (CVB3) are considered to be the most common etiologic agents of viral myocarditis. There are not any special anti CVB3 drugs yet.From previous studies, the anti CVB3 affect of sophora flavescens Ait (SFA) had been discovered. Our experiment was to study the anti CVB3 ability of SFA to cultured beating myocardial cells of CVB3 infected newborn rat.Methods The myocardial cells were divided into four groups:①Infected group (n=8),infected only with CVB3, not adding SFA;② Treated group (n=8),infected with CVB3, adding SFA (100 μg/ml);③Drug group (n=6),adding SFA (100 μg/ml) only;④Control group (n=6),not infected with CVB3, not adding SFA.Results The myocardial cells of the infected group had cell pathogenic effect (CPE) on the second day after virus inoculation, the CPE progressed rapidly from+to++++. In contrast, no CPE in the other three groups was found. The LDH and SGOT of the infected group were higher than that in the other three groups, showing a significant difference (P<0.05).The virus titer of the infected group was higher than that of the treated group. There was no influence on normal myocardial cells if the concentration of SFA was lower than 300 μg/ml. When the concentration of SFA was 6.25 μg/ml～200 μg/ml, it showed protective effect on infected myocardial cells.Conclusion The results of the experiment suggest that SFA might inhibit CVB3 replication in myocardial cells.

关 键 词：柯萨奇病毒B组 苦参 病毒性心肌炎 

分 类 号：R285[医药卫生—中药学]